Difference between revisions of "SigM"

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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
 +
** expression of [[CsbB]] in the absence of [[YfhO]] causes constitutive activation of [[SigM]] {{PubMed|23632331}}
 +
** shortage of available bactoprenol causes [[SigM]] activation {{PubMed|23632331}}
  
 
* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
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* '''Additional information:'''
 
* '''Additional information:'''
 +
** expression of [[CsbB]] in the absence of [[YfhO]] causes constitutive activation of [[SigM]] {{PubMed|23632331}}
 +
** shortage of available bactoprenol causes [[SigM]] activation {{PubMed|23632331}}
  
 
=Biological materials =
 
=Biological materials =
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=References=
 
=References=
'''Additional publications:''' {{PubMed|23070162,22211522,20817771}}
 
 
==The [[SigM regulon]]==
 
==The [[SigM regulon]]==
 
<pubmed>18179421,17675383,17434969,</pubmed>
 
<pubmed>18179421,17675383,17434969,</pubmed>
 
==Other original publications==
 
==Other original publications==
<pubmed>22362028 18394148,14993308,12399481,12775685,12207695,18820022,15838020,19047346,14651641,16151214 ,19465659  19745567 10216858 20057163 23103977 </pubmed>
+
<pubmed>22362028 18394148,14993308,12399481,12775685,12207695,18820022,15838020,19047346,14651641,16151214 ,19465659  19745567 10216858 20057163 23103977 23632331 23070162,22211522,20817771</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 18:14, 6 May 2013

Gene name sigM
Synonyms yhdM
Essential no
Product RNA polymerase ECF-type sigma factor SigM
Function responsible for intrinsic resistance against beta-lactam antibiotics
Gene expression levels in SubtiExpress: sigM
Interactions involving this protein in SubtInteract: SigM
MW, pI 19 kDa, 6.77
Gene length, protein length 489 bp, 163 aa
Immediate neighbours yhdL, yhdN
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SigM context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SigM expression.png















Categories containing this gene/protein

transcription, sigma factors and their control, cell envelope stress proteins (controlled by SigM, V, W, X, Y), resistance against toxins/ antibiotics

This gene is a member of the following regulons

SigM regulon

The SigM regulon

The gene

Basic information

  • Locus tag: BSU09520

Phenotypes of a mutant

  • SigM is essential for growth and survival in nutrient broth (NB) containing 1.4 M NaCl PubMed
  • sigM mutants form aberrantly shaped cells, which swell and lyse spontaneously during growth in NB medium containing increased levels (0.35-0.7 M) of a wide range of different salts PubMed
  • increased sensitivity towards beta-lactam antibiotics PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: sigma-70 factor family, ECF subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • expression of CsbB in the absence of YfhO causes constitutive activation of SigM PubMed
    • shortage of available bactoprenol causes SigM activation PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:
    • expression of CsbB in the absence of YfhO causes constitutive activation of SigM PubMed
    • shortage of available bactoprenol causes SigM activation PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage

Your additional remarks

References

The SigM regulon

Other original publications