TasA

• Description: major component of biofilm matrix, forms amyloid fibers
  
  

Gene name	tasA
Synonyms	cotN, yqhF
Essential	no
Product	major component of biofilm matrix
Function	biofilm formation
Gene expression levels in SubtiExpress: tasA
Interactions involving this protein in SubtInteract: TasA
Regulation of this protein in SubtiPathways: Biofilm, Protein secretion
MW, pI	28 kDa, 5.442
Gene length, protein length	783 bp, 261 aa
Immediate neighbours	sinR, sipW
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biofilm formation

This gene is a member of the following regulons

AbrB regulon, SinR regulon

The gene

Basic information

• Locus tag: BSU24620

Phenotypes of a mutant

• altered cell death pattern in colonies PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: forms amyloid fibers that bind cells together in the biofilm PubMed

• Protein family: peptidase M73 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • forms fibers of variable length and 10-15 nm in width PubMed
  • TapA-TasA PubMed
  • SipW-TasA PubMed

• Localization:
  • extracellular (signal peptide) PubMed, secretion requires SipW PubMed
  • membrane PubMed

Database entries

• Structure:

• UniProt: P54507

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: tapA-sipW-tasA PubMed

• Expression browser: tasA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed
  • repressed by SinR PubMed
  • repressed during logrithmic growth (AbrB) PubMed

• Regulatory mechanism:
  • AbrB: transcription repression PubMed
  • SinR: transcription repression PubMed

• Additional information:
  • induction by sequestration of SinR by SinI or SlrA PubMed or by SlrR PubMed
  • the tapA-sipW-tasA operon is not expressed in a ymdB mutant PubMed
  • the amount of the mRNA is substantially decreased upon depletion of RNase Y (this is likely due to the increased stability of the sinR mRNA) PubMed

Biological materials

• Mutant:
  • 1S121 ( tasA::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: