TatAD

• Description: component of the TatAD-TatCD twin-arginine translocase
  
  

Gene name	tatAD
Synonyms	yczB
Essential	no
Product	component of the twin-arginine translocation pathway
Function	TAT protein secretion
Gene expression levels in SubtiExpress: tatAD
Interactions involving this protein in SubtInteract: TatAD
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	7 kDa, 9.693
Gene length, protein length	210 bp, 70 aa
Immediate neighbours	phoD, tatCD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

phosphate metabolism, protein secretion, membrane proteins

This gene is a member of the following regulons

PhoP regulon

The gene

Basic information

• Locus tag: BSU02630

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: translocation of PhoD PubMed

• Protein family: tatA/E family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • TatAD-PhoD PubMed
  • TatAD-TatCD, TatAD-TatCY PubMed
  • CsbC-TatAD PubMed
  • TatAC-TatAD PubMed
  • TatAY-TatAY PubMed

• Localization: membrane (according to Swiss-Prot), forms foci at the division sites and cell poles, localization requires interaction with TatCD or TatCY PubMed

Database entries

• Structure: 2L16 PubMed

• UniProt: O31467

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: phoD-tatAD-tatCD PubMed

• Expression browser: tatAD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions of phosphate limitation (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription activation PubMed

• Additional information:
  • expression of the operon depends on functional HemAT and CsbC PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Oscar Kuipers, University of Groningen, The Netherlands Homepage

References

Reviews

Tracy Palmer, Ben C Berks
The twin-arginine translocation (Tat) protein export pathway.
Nat Rev Microbiol: 2012, 10(7);483-96
[PubMed:22683878] [WorldCat.org] [DOI] (I e)