LicT

• Description: transcriptional antiterminator of the bglP-bglH-yxiE operon and the bglS gene
  
  

Gene name	licT
Synonyms
Essential	no
Product	transcriptional antiterminator (BglG family)
Function	control of beta-glucan and beta-glucoside utilization
Gene expression levels in SubtiExpress: licT
Interactions involving this protein in SubtInteract: LicT
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	32 kDa, 5.944
Gene length, protein length	831 bp, 277 aa
Immediate neighbours	bglS, yxiP
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control, RNA binding regulators, phosphoproteins

The gene

Basic information

• Locus tag: BSU39080

Phenotypes of a mutant

no expression of the bglP-bglH operon

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binding to the mRNAs of bglS and the bglP-bglH operon, causes transcription antitermination (in presence of salicin and absence of glucose)

• Protein family: transcriptional antiterminator BglG family of antiterminators (according to Swiss-Prot)

• Paralogous protein(s): SacY, GlcT, SacT

Extended information on the protein

• Kinetic information:
  • K(D) for the RAT-RNA: 10 nM PubMed
• Domains:
  • N-terminal RNA binding domain Pubmed
  • 2xPRD (PTS regulation domains) PubMed

• Modification:
  • phosphorylation at His-100 in PRD-1 by phosphorylated BglP, inhibits LicT antitermination activity
  • phosphorylation at His-207 and/or His-269 in PRD-2 by His-P-HPr, stimulates LicT antitermination activity

• Cofactor(s):

• Effectors of protein activity:

• Interactions: PtsH-LicT, BglP-LicT

• Localization:
  • cytoplasm, even distribution in the absence of the inducer salicin, subpolar localization in the presence of salicin PubMed

Database entries

• Structure: 1L1C (complex with RAT), 1TLV (PRDs)

• UniProt: P39805

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: licT-bglS PubMed

• Expression browser: licT PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP427 (licTS, erm), available in the Stülke lab

• Expression vector:

• • for expression, purification of both PRDs in E. coli with N-terminal His-tag, in pWH844: pGP165, available in Stülke lab
  • for expression, purification of the RNA-binding domain in E. coli with N-terminal His-tag, in pWH844: pGP315, available in Stülke lab
  • for expression, purification of the RNA-binding domain in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP572, available in Stülke lab

• lacZ fusion:

• GFP fusion: GP1225 (spc, based on pGP1870), available in the Stülke lab

• YFP fusion: GP1229 (spc, based on pGP1871), available in the Stülke lab

• FLAG-tag construct: GP1221 (spc, based on pGP1331), available in the Stülke lab

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

Josef Deutscher, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

Michael Hecker, Greifswald, Germany Homepage

References

Additional publications: PubMed

Original description

Control of LicT activity

Fabian M Rothe, Christoph Wrede, Martin Lehnik-Habrink, Boris Görke, Jörg Stülke  
Dynamic localization of a transcription factor in Bacillus subtilis:
The LicT antiterminator relocalizes in response to inducer availability. 
J Bacteriol.: 2013, 195, Epub ahead of print. 
PubMed:23475962

Structural analysis of LicT

LicT-RNA interaction