Difference between revisions of "Rnc"
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| − | + | * essential {{PubMed|23300471,12682299,11123676}} | |
| − | essential | + | * the ''[[rnc]]'' gene can be deleted in strains cured of the [[Skin element]] and [[prophage SPß]] (or upon deletion of the toxin genes ''[[txpA]]'' and ''[[yonT]]'') {{PubMed|23300471}} |
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=== Database entries === | === Database entries === | ||
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=== Additional information=== | === Additional information=== | ||
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=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot) cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (''[[scr]]'') | + | * '''Catalyzed reaction/ biological activity:''' |
| + | ** Endonucleolytic cleavage of double-stranded RNA to 5'-phosphomonoester (according to Swiss-Prot) | ||
| + | ** cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (''[[scr]]'') | ||
| + | ** degradation of ''[[txpA]]'' mRNA-[[RatA]] RNA duplex (encoded on the [[Skin element]]) {{PubMed|23300471}} | ||
| + | ** degradation of ''[[yonT]]'' mRNA-[[as-YonT]] RNA duplex (encoded on the [[SP-beta prophage]]) {{PubMed|23300471}} | ||
* '''Protein family:''' | * '''Protein family:''' | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rnc_1665710_1666459_1 rnc] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rnc_1665710_1666459_1 rnc] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' [[SigA]] {{PubMed|11021934}} | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|11021934}} |
* '''Regulation:''' | * '''Regulation:''' | ||
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=References= | =References= | ||
==Reviews== | ==Reviews== | ||
| − | <pubmed> 17194582 16179796 8936814 16855311 </pubmed> | + | <pubmed> 17194582 16179796 8936814 16855311 23300472 </pubmed> |
==Original publications== | ==Original publications== | ||
| − | <pubmed>9393702 11123676 7527387 9677377 17576666, 11021934 6409421 22123973 22412379 </pubmed> | + | <pubmed>9393702 11123676 7527387 9677377 17576666, 11021934 6409421 22123973 22412379 23300471 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 13:03, 10 January 2013
- Description: RNase III; required for the degradation of sense/antisense transcripts in Gram positive, low GC bacteria
| Gene name | rnc |
| Synonyms | rncS |
| Essential | yes |
| Product | endoribonuclease III |
| Function | cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr) |
| Gene expression levels in SubtiExpress: rnc | |
| Metabolic function and regulation of this protein in SubtiPathways: Protein secretion | |
| MW, pI | 28 kDa, 8.076 |
| Gene length, protein length | 747 bp, 249 aa |
| Immediate neighbours | acpA, smc |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
Rnases, translation, protein secretion, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU15930
Phenotypes of a mutant
- essential PubMed
- the rnc gene can be deleted in strains cured of the Skin element and prophage SPß (or upon deletion of the toxin genes txpA and yonT) PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Endonucleolytic cleavage of double-stranded RNA to 5'-phosphomonoester (according to Swiss-Prot)
- cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr)
- degradation of txpA mRNA-RatA RNA duplex (encoded on the Skin element) PubMed
- degradation of yonT mRNA-as-YonT RNA duplex (encoded on the SP-beta prophage) PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P51833
- KEGG entry: [3]
- E.C. number: 3.1.26.3
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
David Bechhofer, Mount Sinai School, New York, USA Homepage
Ciaran Condon, IBPC, Paris, France Homepage
Your additional remarks
References
Reviews
Original publications
