Difference between revisions of "SkfA"
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU01910 skfA] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU01910 skfA] | ||
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| + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SkfA SkfA] | ||
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 5 kDa, 10.648 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 5 kDa, 10.648 | ||
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* '''Domains:''' | * '''Domains:''' | ||
| − | * '''Modification:''' the mature SKF is cyclic 26-amino acid peptide that is posttranslationally modified with one disulfide and one cysteine thioether bridged to the alpha-position of a methionine {{PubMed|20805502}} | + | * '''Modification:''' the mature SKF is cyclic 26-amino acid peptide that is posttranslationally modified with one disulfide and one cysteine thioether bridged to the alpha-position of a methionine {{PubMed|20805502}}, the thioether bond is generated by [[SkfB]] {{PubMed|23282011}} |
* '''Cofactor(s):''' | * '''Cofactor(s):''' | ||
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* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
| + | ** [[SkfA]]-[[SkfB]] {{PubMed|23282011}} | ||
* '''[[Localization]]:''' secreted (according to Swiss-Prot) | * '''[[Localization]]:''' secreted (according to Swiss-Prot) | ||
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<pubmed>20955377 </pubmed> | <pubmed>20955377 </pubmed> | ||
==Original Publications== | ==Original Publications== | ||
| − | <pubmed>12884008,15812018,14651647,15687200, 17720793, 12850135,12817086,16816204,17449691,18840696 | + | '''Additional publications:''' {{PubMed|23282011,20805502}} |
| + | <pubmed>12884008,15812018,14651647,15687200, 17720793, 12850135,12817086,16816204,17449691,18840696 </pubmed> | ||
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[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 12:24, 4 January 2013
- Description: spore killing factor
| Gene name | skfA |
| Synonyms | ybcO |
| Essential | no |
| Product | spore killing factor |
| Function | killing of sister cells |
| Gene expression levels in SubtiExpress: skfA | |
| Interactions involving this protein in SubtInteract: SkfA | |
| MW, pI | 5 kDa, 10.648 |
| Gene length, protein length | 165 bp, 55 aa |
| Immediate neighbours | ybcM, skfB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
toxins, antitoxins and immunity against toxins
This gene is a member of the following regulons
AbrB regulon, PhoP regulon, Spo0A regulon
The gene
Basic information
- Locus tag: BSU01910
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: the mature SKF is cyclic 26-amino acid peptide that is posttranslationally modified with one disulfide and one cysteine thioether bridged to the alpha-position of a methionine PubMed, the thioether bond is generated by SkfB PubMed
- Cofactor(s):
- Effectors of protein activity:
- Localization: secreted (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O31422
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
- Northern blotting during during phosphate limitation showed an intense 0.25 kb skfA-specific transcript, and a weaker 6.5 kb skfA-skfB-skfC-skfE-skfF-skfG-skfH transcript.
- The skfA transcript is stabilized by a stem-loop structure in the skfA-skfB intergenic region. PubMed
- The mRNA is very stable (half-life > 15 min) PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
José Eduardo González-Pastor
Cannibalism: a social behavior in sporulating Bacillus subtilis.
FEMS Microbiol Rev: 2011, 35(3);415-24
[PubMed:20955377]
[WorldCat.org]
[DOI]
(I p)
Original Publications
Additional publications: PubMed
