MtlR

• Description: transcriptional activator of the mtlA-mtlF-mtlD operon
  
  

Gene name	mtlR
Synonyms	ydaA
Essential	no
Product	transcriptional activator, PRD-type
Function	regulation of mannitol utilization
Gene expression levels in SubtiExpress: mtlR
Interactions involving this protein in SubtInteract: MtlR
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	78 kDa, 5.313
Gene length, protein length	2082 bp, 694 aa
Immediate neighbours	ycsN, ydaB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control, phosphoproteins

This gene is a member of the following regulons

CcpA regulon

The MtlR regulon:

• mtlA-mtlF-mtlD

The gene

Basic information

• Locus tag: BSU04160

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: N-terminal DNA-binding domains, two PTS-regulation domains (PRD1 and PRD2), EIIB (Gat)-like domain, EIIA (Mtl)-like domain

• Modification:
  • phosphorylated on His-342 and/or His-399 by PtsH PubMed
  • phosphorylation on His-599 by MtlA PubMed
  • phosphorylation on Cys-419 by MtlF PubMed

• Cofactor(s):

• Effectors of protein activity:
  • activity is stimulated by PtsH-dependent phosphorylation in PRD2 (mechnism of carbon catabolite repression) PubMed
  • activity is inhibited by MtlF-dependent phosphorylation in the EIIB(Gat)-like domain on Cys-419 (this prevents activity in the absence of mannitol and allows induction in presence of mannitol) PubMed

• Interactions:
  • PtsH-MtlR PubMed
  • MtlA-MtlR PubMed (the non-phosphorylated IIB domain of MtlA interacts with the two C-terminal EIIB (Gat)-like and EIIA (Mtl)-like domains of MtlR) PubMed
  • MtlF-MtlR PubMed

• Localization:
  • upon interaction with non-phosphorylated MtlA, the active MtlR localizes to the membrane PubMed

Database entries

• Structure:

• UniProt: P96574

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mtlR PubMed

• Expression browser: mtlR PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.
BMC Genomics: 2012, 13;401
[PubMed:22900538] [WorldCat.org] [DOI] (I e)