Difference between revisions of "CshA"
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
{{SubtiWiki category|[[DEAD-box RNA helicases]]}}, | {{SubtiWiki category|[[DEAD-box RNA helicases]]}}, | ||
| + | {{SubtiWiki category|[[translation]]}}, | ||
{{SubtiWiki category|[[cold stress proteins]]}}, | {{SubtiWiki category|[[cold stress proteins]]}}, | ||
{{SubtiWiki category|[[membrane proteins]]}} | {{SubtiWiki category|[[membrane proteins]]}} | ||
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | * poor growth at low temperatures (16 to 20°C) {{PubMed|23175651}} | ||
| + | * reduced number of ribosomes {{PubMed|23175651}} | ||
=== Database entries === | === Database entries === | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cshA_511106_512641_1 cshA] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cshA_511106_512641_1 cshA] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
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=References= | =References= | ||
'''Additional publications:''' {{PubMed|20709848,21710567}} | '''Additional publications:''' {{PubMed|20709848,21710567}} | ||
| − | <pubmed>16352840,20572937 , 12399512 16861794, 21764917 ,21803996</pubmed> | + | <pubmed>16352840,20572937 , 12399512 16861794, 21764917 23175651,21803996</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 13:00, 24 November 2012
- Description: DEAD-box RNA helicase, important for adaptation to low temperatures
| Gene name | cshA |
| Synonyms | ydbR |
| Essential | no |
| Product | DEAD-box RNA helicase |
| Function | RNA helicase |
| Gene expression levels in SubtiExpress: cshA | |
| Interactions involving this protein in SubtInteract: CshA | |
| MW, pI | 57 kDa, 9.89 |
| Gene length, protein length | 1533 bp, 511 aa |
| Immediate neighbours | murF, ydbS |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
[None Expression at a glance] PubMed
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Contents
Categories containing this gene/protein
DEAD-box RNA helicases, translation, cold stress proteins, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU04580
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: RNA helicase
- Protein family: helicase C-terminal domain (according to Swiss-Prot) DEAD-box RNA helicase
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasma, colocalizes with the ribosomes PubMed, cell membrane PubMed
Database entries
- Structure:
- UniProt: P96614
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP1035 (aphA3), available in Jörg Stülke's lab
- GP1083 (cat), available in Jörg Stülke's lab
- Expression vector:
- for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, in pGP382: pGP1387, available in Jörg Stülke's lab
- for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1026 (aphA3), available in Jörg Stülke's lab
- for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP1386, available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- pGP1369 for chromosomal expression of CshA-YFP, available in Jörg Stülke's lab
- B. subtilis GP1081 cshA-gfp spc, available in Jörg Stülke's lab,
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- FLAG-tag construct:
- GP1010 (spc, based on pGP1331), available in Jörg Stülke's lab
- GP1074 (tet), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Mohamed Marahiel, Marburg University, Germany homepage
Your additional remarks
References
Additional publications: PubMed
Martin Lehnik-Habrink, Leonie Rempeters, Ákos T Kovács, Christoph Wrede, Claudia Baierlein, Heike Krebber, Oscar P Kuipers, Jörg Stülke
DEAD-Box RNA helicases in Bacillus subtilis have multiple functions and act independently from each other.
J Bacteriol: 2013, 195(3);534-44
[PubMed:23175651]
[WorldCat.org]
[DOI]
(I p)
Martin Lehnik-Habrink, Joseph Newman, Fabian M Rothe, Alexandra S Solovyova, Cecilia Rodrigues, Christina Herzberg, Fabian M Commichau, Richard J Lewis, Jörg Stülke
RNase Y in Bacillus subtilis: a Natively disordered protein that is the functional equivalent of RNase E from Escherichia coli.
J Bacteriol: 2011, 193(19);5431-41
[PubMed:21803996]
[WorldCat.org]
[DOI]
(I p)
Christelle M Roux, Jonathon P DeMuth, Paul M Dunman
Characterization of components of the Staphylococcus aureus mRNA degradosome holoenzyme-like complex.
J Bacteriol: 2011, 193(19);5520-6
[PubMed:21764917]
[WorldCat.org]
[DOI]
(I p)
Martin Lehnik-Habrink, Henrike Pförtner, Leonie Rempeters, Nico Pietack, Christina Herzberg, Jörg Stülke
The RNA degradosome in Bacillus subtilis: identification of CshA as the major RNA helicase in the multiprotein complex.
Mol Microbiol: 2010, 77(4);958-71
[PubMed:20572937]
[WorldCat.org]
[DOI]
(I p)
Yoshinari Ando, Kouji Nakamura
Bacillus subtilis DEAD protein YdbR possesses ATPase, RNA binding, and RNA unwinding activities.
Biosci Biotechnol Biochem: 2006, 70(7);1606-15
[PubMed:16861794]
[WorldCat.org]
[DOI]
(P p)
Karen Hunger, Carsten L Beckering, Frank Wiegeshoff, Peter L Graumann, Mohamed A Marahiel
Cold-induced putative DEAD box RNA helicases CshA and CshB are essential for cold adaptation and interact with cold shock protein B in Bacillus subtilis.
J Bacteriol: 2006, 188(1);240-8
[PubMed:16352840]
[WorldCat.org]
[DOI]
(P p)
Carsten L Beckering, Leif Steil, Michael H W Weber, Uwe Völker, Mohamed A Marahiel
Genomewide transcriptional analysis of the cold shock response in Bacillus subtilis.
J Bacteriol: 2002, 184(22);6395-402
[PubMed:12399512]
[WorldCat.org]
[DOI]
(P p)
