Pta

• Description: phosphotransacetylase
  
  

Gene name	pta
Synonyms	ipa-88d, ywfJ
Essential	no
Product	phosphotransacetylase
Function	overflow metabolism
Gene expression levels in SubtiExpress: pta
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	34 kDa, 4.649
Gene length, protein length	969 bp, 323 aa
Immediate neighbours	cysL, ywfI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, phosphoproteins

This gene is a member of the following regulons

CcpA regulon

The gene

Basic information

• Locus tag: BSU37660

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Acetyl-CoA + phosphate = CoA + acetyl phosphate (according to Swiss-Prot)

• Protein family: phosphate acetyltransferase and butyryltransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on (Ser-123 OR Thr-128 OR Ser-129) PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1XCO (complex with acetylphosphate), 1TD9

• UniProt: P39646

• KEGG entry: [3]

• E.C. number: 2.3.1.8

Expression and regulation

• Operon: pta PubMed

• Expression browser: pta PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expression activated by glucose (2.6 fold) (CcpA) PubMed

• Regulatory mechanism:
  • CcpA: transcription activation PubMed

• Additional information:

Biological materials

• Mutant: GP668 (aphA3), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Alan J Wolfe
The acetate switch.
Microbiol Mol Biol Rev: 2005, 69(1);12-50
[PubMed:15755952] [WorldCat.org] [DOI] (P p)

Original publications

Additional publications: PubMed

V K Morya, Varun Dewaker, Eun-Ki Kim
In silico study and validation of phosphotransacetylase (PTA) as a putative drug target for Staphylococcus aureus by homology-based modelling and virtual screening.
Appl Biochem Biotechnol: 2012, 168(7);1792-805
[PubMed:23054816] [WorldCat.org] [DOI] (I p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

Qian Steven Xu, Jarmila Jancarik, Yun Lou, Kate Kuznetsova, Alexander F Yakunin, Hisao Yokota, Paul Adams, Rosalind Kim, Sung-Hou Kim
Crystal structures of a phosphotransacetylase from Bacillus subtilis and its complex with acetyl phosphate.
J Struct Funct Genomics: 2005, 6(4);269-79
[PubMed:16283428] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

E Presecan-Siedel, A Galinier, R Longin, J Deutscher, A Danchin, P Glaser, I Martin-Verstraete
Catabolite regulation of the pta gene as part of carbon flow pathways in Bacillus subtilis.
J Bacteriol: 1999, 181(22);6889-97
[PubMed:10559153] [WorldCat.org] [DOI] (P p)

B S Shin, S K Choi, S H Park
Regulation of the Bacillus subtilis phosphotransacetylase gene.
J Biochem: 1999, 126(2);333-9
[PubMed:10423526] [WorldCat.org] [DOI] (P p)