MetI

• Description: O-succinylhomoserine lyase (L-cysteine, H2S, methanethiol, elimination)
  
  

Gene name	metI
Synonyms	yjcI
Essential	no
Product	O-succinylhomoserine lyase (L-cysteine, H2S, methanethiol, elimination)
Function	biosynthesis of methionine
Gene expression levels in SubtiExpress: metI
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	41 kDa, 5.019
Gene length, protein length	1119 bp, 373 aa
Immediate neighbours	yjcH, metC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

S-box

The gene

Basic information

• Locus tag: BSU11870

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O31631

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: metI-metC PubMed

• Expression browser: metI PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed
  • induced by methionine starvation (S-box) PubMed

• Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A941 ( metI::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: