RocR

• Description: transcriptional activator of arginine utilization operons
  
  

Gene name	rocR
Synonyms
Essential	no
Product	transcriptional activator
Function	transcriptional activator of arginine utilization operons
Gene expression levels in SubtiExpress: rocR
Interactions involving this protein in SubtInteract: RocR
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	52.6 kDa, 6.08
Gene length, protein length	1383 bp, 461 amino acids
Immediate neighbours	rocD, yyxA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

• utilization of amino acids,

transcription factors and their control

• see also: glutamate metabolism

The gene

Basic information

• Locus tag: BSU40350

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transcription activation at SigL-dependent promoters of rocABC, rocDEF, and rocG

• Protein family: Sigma-54 interacting transcription activator

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • Sigma-54 factor interaction domain (143–372)
  • 2x nucleotid-binding domain (171–178),(233–242)
  • HTH motif (434–453)

• Modification:

• Cofactor(s): ornithine or citrulline are required for RocR-dependent transcription activation PubMed

• Effectors of protein activity:

• Interactions:
  • RocR-SigL

• Localization:

Database entries

• Structure:

• UniProt: P38022

• KEGG entry: [3]

Expression and regulation

• Operon: rocR (according to DBTBS)

• Expression browser: rocR PubMed

• Sigma factor: SigA PubMed

• Regulation: autoregulation by RocR PubMed

• Regulatory mechanism: autorepression

• Additional information:

Biological materials

• Mutant: QB5533 (aphA3), from Michel Debarbouille, available in Stülke lab
  • 1A911 ( rocR::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• Antibody:

Labs working on this gene/protein

Michel Debarbouille, Pasteur Institute, Paris, France Homepage