KinC

• Description: two-component sensor kinase, phosphorylates Spo0F and Spo0A in response to the presence of surfactin, part of the phosphorelay, governs expression of genes involved in biofilm formation
  
  

Gene name	kinC
Synonyms	ssb
Essential	no
Product	two-component sensor kinase
Function	initiation of sporulation
Gene expression levels in SubtiExpress: kinC
Interactions involving this protein in SubtInteract: KinC
Function and regulation of this protein in SubtiPathways: Phosphorelay
MW, pI	48 kDa, 6.225
Gene length, protein length	1284 bp, 428 aa
Immediate neighbours	abh, ykqA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, transcription factors and their control, phosphorelay, biofilm formation, membrane proteins, phosphoproteins

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

• Locus tag: BSU14490

Phenotypes of a mutant

• • defective in biofilm formation PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • autophosphorylation, phosphorylation of Spo0F as part of the phosphorelay, but also direct phosphorylation of Spo0A PubMed
  • mainly active in the younger, outer regions of a colony (with KinD) PubMed
  • phosphorylates Spo0A in response to the presence of surfactin PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: two transmembrane segments, C-terminal histidine phosphotransferase domain

• Modification: autophosphorylation on a His residue

• Cofactor(s):

• Effectors of protein activity:
  • activity is triggered by potassium leakage PubMed
  • activity is triggered by polyisoprenoid lipids formed by YisP PubMed

• Interactions:
  • KinC-Spo0A PubMed
  • KinC-Spo0F

• Localization:
  • cell membrane (Heterogeneous) PubMed
  • co-localizes with FloT in discrete foci in the membrane PubMed
  • the localization of KinC in membrane microdomains depends on FloA and FloT PubMed

Database entries

• Structure:

• UniProt: P39764

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: kinC (according to DBTBS)

• Expression browser: kinC PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A632 ( kinC::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: