Ald

• Description: L-alanine dehydrogenase
  
  

Gene name	ald
Synonyms	spoVN
Essential	no
Product	L-alanine dehydrogenase
Function	alanine utilization
Metabolic function and regulation of this protein in SubtiPathways: Ala, Gly, Ser
MW, pI	39 kDa, 5.125
Gene length, protein length	1134 bp, 378 aa
Immediate neighbours	adeR, yukJ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

AdeR regulon

The gene

Basic information

• Locus tag: BSU31930

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-alanine + H₂O + NAD⁺ = pyruvate + NH₃ + NADH (according to Swiss-Prot)

• Protein family: AlaDH/PNT family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2EEZ (from Thermus thermophilus hb8 mutant, 55% identity, 66% similarity)

• UniProt: Q08352

• KEGG entry: [3]

• E.C. number: 1.4.1.1

Expression and regulation

• Operon: ald PubMed

• Expression browser: ald PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by alanine (AdeR) PubMed

• Regulatory mechanism:
  • AdeR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ta-Hui Lin, Guei-Tsung Wei, Chien-Chen Su, Gwo-Chyuan Shaw
AdeR, a PucR-type transcription factor, activates expression of L-alanine dehydrogenase and is required for sporulation of Bacillus subtilis.
J Bacteriol: 2012, 194(18);4995-5001
[PubMed:22797752] [WorldCat.org] [DOI] (I p)

Jessica C Wilks, Ryan D Kitko, Sarah H Cleeton, Grace E Lee, Chinagozi S Ugwu, Brian D Jones, Sandra S BonDurant, Joan L Slonczewski
Acid and base stress and transcriptomic responses in Bacillus subtilis.
Appl Environ Microbiol: 2009, 75(4);981-90
[PubMed:19114526] [WorldCat.org] [DOI] (I p)

Le Thi Tam, Christine Eymann, Haike Antelmann, Dirk Albrecht, Michael Hecker
Global gene expression profiling of Bacillus subtilis in response to ammonium and tryptophan starvation as revealed by transcriptome and proteome analysis.
J Mol Microbiol Biotechnol: 2007, 12(1-2);121-30
[PubMed:17183219] [WorldCat.org] [DOI] (P p)

Bauke Oudega, Micheline Vandenbol, Gregory Koningstein
A 12 kb nucleotide sequence containing the alanine dehydrogenase gene at 279 degrees on the Bacillus subtilis chromosome.
Microbiology (Reading): 1997, 143 ( Pt 5);1489-1491
[PubMed:9168598] [WorldCat.org] [DOI] (P p)

K J Siranosian, K Ireton, A D Grossman
Alanine dehydrogenase (ald) is required for normal sporulation in Bacillus subtilis.
J Bacteriol: 1993, 175(21);6789-96
[PubMed:8226620] [WorldCat.org] [DOI] (P p)