Spo0E

• Description: Spo0A-P phosphatase, control of the phosphorelay
  
  

Gene name	spo0E
Synonyms
Essential	no
Product	Spo0A-P phosphatase
Function	initiation of sporulation
Interactions involving this protein in SubtInteract: Spo0E
Function and regulation of this protein in SubtiPathways: Phosphorelay
MW, pI	9 kDa, 6.483
Gene length, protein length	255 bp, 85 aa
Immediate neighbours	ykvA, eag
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, phosphorelay

This gene is a member of the following regulons

SigB regulon, AbrB regulon, Rok regulon

The gene

Basic information

• Locus tag: BSU13640

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: spo0E family (according to Swiss-Prot)

• Paralogous protein(s): YnzD

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • Spo0F-Spo0E PubMed
  • Spo0A-Spo0E PubMed

• Localization:

Database entries

• Structure:

• UniProt: P05043

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spo0E PubMed

• Expression browser: spo0E PubMed

• Sigma factor:
  • SigA PubMed, SigB PubMed

• Regulation:
  • repressed during logrithmic growth (AbrB) PubMed
  • induced under stress conditions (SigB) PubMed

• Regulatory mechanism:
  • AbrB: transcription repression PubMed
  • Rok: transcription repression of the SigB-dependent promoter PubMed

• Additional information:
  • Spo0E is degraded by FtsH
  • stress-induced expression of Spo0E by SigB results in prevention of sporulation during stress (due to dephosphorylation of Spo0A) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Tony Wilkinson, York University, U.K. homepage

References

Reviews

Daniel Schultz, Peter G Wolynes, Eshel Ben Jacob, José N Onuchic
Deciding fate in adverse times: sporulation and competence in Bacillus subtilis.
Proc Natl Acad Sci U S A: 2009, 106(50);21027-34
[PubMed:19995980] [WorldCat.org] [DOI] (I p)

Original Publications

Additional publications: PubMed

Ai Thi Thuy Le, Wolfgang Schumann
The Spo0E phosphatase of Bacillus subtilis is a substrate of the FtsH metalloprotease.
Microbiology (Reading): 2009, 155(Pt 4);1122-1132
[PubMed:19332814] [WorldCat.org] [DOI] (P p)

Shigeo Hosoya, Kei Asai, Naotake Ogasawara, Michio Takeuchi, Tsutomu Sato
Mutation in yaaT leads to significant inhibition of phosphorelay during sporulation in Bacillus subtilis.
J Bacteriol: 2002, 184(20);5545-53
[PubMed:12270811] [WorldCat.org] [DOI] (P p)

Andrea Feucht, Laura Abbotts, Jeffery Errington
The cell differentiation protein SpoIIE contains a regulatory site that controls its phosphatase activity in response to asymmetric septation.
Mol Microbiol: 2002, 45(4);1119-30
[PubMed:12180929] [WorldCat.org] [DOI] (P p)

Sophie J Stephenson, Marta Perego
Interaction surface of the Spo0A response regulator with the Spo0E phosphatase.
Mol Microbiol: 2002, 44(6);1455-67
[PubMed:12067336] [WorldCat.org] [DOI] (P p)

H Nanamiya, K Takahashi, M Fujita, F Kawamura
Deficiency of the initiation events of sporulation in Bacillus subtilis clpP mutant can be suppressed by a lack of the Spo0E protein phosphatase.
Biochem Biophys Res Commun: 2000, 279(1);229-33
[PubMed:11112444] [WorldCat.org] [DOI] (P p)

I Lucet, A Feucht, M D Yudkin, J Errington
Direct interaction between the cell division protein FtsZ and the cell differentiation protein SpoIIE.
EMBO J: 2000, 19(7);1467-75
[PubMed:10747015] [WorldCat.org] [DOI] (P p)

K L Ohlsen, J K Grimsley, J A Hoch
Deactivation of the sporulation transcription factor Spo0A by the Spo0E protein phosphatase.
Proc Natl Acad Sci U S A: 1994, 91(5);1756-60
[PubMed:8127878] [WorldCat.org] [DOI] (P p)

M Perego, J A Hoch
Negative regulation of Bacillus subtilis sporulation by the spo0E gene product.
J Bacteriol: 1991, 173(8);2514-20
[PubMed:1901567] [WorldCat.org] [DOI] (P p)

U Bai, M Lewandoski, E Dubnau, I Smith
Temporal regulation of the Bacillus subtilis early sporulation gene spo0F.
J Bacteriol: 1990, 172(9);5432-9
[PubMed:2118512] [WorldCat.org] [DOI] (P p)

M A Strauch, G B Spiegelman, M Perego, W C Johnson, D Burbulys, J A Hoch
The transition state transcription regulator abrB of Bacillus subtilis is a DNA binding protein.
EMBO J: 1989, 8(5);1615-21
[PubMed:2504584] [WorldCat.org] [DOI] (P p)

M Perego, J A Hoch
Isolation and sequence of the spo0E gene: its role in initiation of sporulation in Bacillus subtilis.
Mol Microbiol: 1987, 1(1);125-32
[PubMed:2838724] [WorldCat.org] [DOI] (P p)