Difference between revisions of "Ald"

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(DgLvdJoTXz)
(Undo revision 157744 by 91.201.64.4 (talk))
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NDH8wq  <a href="http://irfsjefqmfjq.com/">irfsjefqmfjq</a>, [url=http://jgenqmspgcar.com/]jgenqmspgcar[/url], [link=http://anrcnpxqlycn.com/]anrcnpxqlycn[/link], http://ijiqzxcextso.com/
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* '''Description:''' L-alanine dehydrogenase <br/><br/>
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{| align="right" border="1" cellpadding="2"
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|-
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|style="background:#ABCDEF;" align="center"|'''Gene name'''
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|''ald''
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|-
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''spoVN ''
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|-
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no
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|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || L-alanine dehydrogenase
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|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || alanine utilization
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/ala_gly_ser.html Ala, Gly, Ser]'''
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|-
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 39 kDa, 5.125 
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|-
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1134 bp, 378 aa
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|-
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yukF]]'', ''[[yukJ]]''
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15181&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|-
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|colspan="2" | '''Genetic context''' <br/> [[Image:ald_context.gif]]
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<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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|-
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|}
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__TOC__
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<br/><br/><br/><br/><br/><br/>
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= [[Categories]] containing this gene/protein =
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{{SubtiWiki category|[[utilization of amino acids]]}}
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 +
= This gene is a member of the following [[regulons]] =
 +
 
 +
 
 +
=The gene=
 +
 
 +
=== Basic information ===
 +
 
 +
* '''Locus tag:''' BSU31930
 +
 
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===Phenotypes of a mutant ===
 +
 
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=== Database entries ===
 +
 
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ald.html]
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* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10468]
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=== Additional information===
 +
 
 +
 
 +
 
 +
 
 +
=The protein=
 +
 
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=== Basic information/ Evolution ===
 +
 
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* '''Catalyzed reaction/ biological activity:''' L-alanine + H<sub>2</sub>O + NAD<sup>+</sup> = pyruvate + NH<sub>3</sub> + NADH (according to Swiss-Prot)
 +
 
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* '''Protein family:''' AlaDH/PNT family (according to Swiss-Prot)
 +
 
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* '''Paralogous protein(s):'''
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=== Extended information on the protein ===
 +
 
 +
* '''Kinetic information:'''
 +
 
 +
* '''Domains:'''
 +
 
 +
* '''Modification:'''
 +
 
 +
* '''Cofactor(s):'''
 +
 
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* '''Effectors of protein activity:'''
 +
 
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* '''[[SubtInteract|Interactions]]:'''
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* '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
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=== Database entries ===
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* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2EEZ 2EEZ] (from ''Thermus thermophilus hb8 mutant'', 55% identity, 66% similarity)
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* '''UniProt:''' [http://www.uniprot.org/uniprot/Q08352 Q08352]
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU31930]
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* '''E.C. number:''' [http://www.expasy.org/enzyme/1.4.1.1 1.4.1.1]
 +
 
 +
=== Additional information===
 +
 
 +
=Expression and regulation=
 +
 
 +
* '''Operon:''' ''[[ald]]'' {{PubMed|8226620}}
 +
 
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* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|8226620}}
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* '''Regulation:'''
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** induced by alanine {{PubMed|8226620}}
 +
 
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* '''Regulatory mechanism:'''
 +
 
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* '''Additional information:'''
 +
 
 +
=Biological materials =
 +
 
 +
* '''Mutant:'''
 +
 
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* '''Expression vector:'''
 +
       
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* '''lacZ fusion:'''
 +
 
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* '''GFP fusion:'''
 +
 
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* '''two-hybrid system:'''
 +
 
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* '''Antibody:'''
 +
 
 +
=Labs working on this gene/protein=
 +
 
 +
=Your additional remarks=
 +
 
 +
=References=
 +
 
 +
<pubmed>19114526,9168598,17183219,8226620,, </pubmed>
 +
 
 +
[[Category:Protein-coding genes]]

Revision as of 13:55, 16 April 2012

  • Description: L-alanine dehydrogenase

Gene name ald
Synonyms spoVN
Essential no
Product L-alanine dehydrogenase
Function alanine utilization
Metabolic function and regulation of this protein in SubtiPathways:
Ala, Gly, Ser
MW, pI 39 kDa, 5.125
Gene length, protein length 1134 bp, 378 aa
Immediate neighbours yukF, yukJ
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Ald context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU31930

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH (according to Swiss-Prot)
  • Protein family: AlaDH/PNT family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2EEZ (from Thermus thermophilus hb8 mutant, 55% identity, 66% similarity)


  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Jessica C Wilks, Ryan D Kitko, Sarah H Cleeton, Grace E Lee, Chinagozi S Ugwu, Brian D Jones, Sandra S BonDurant, Joan L Slonczewski
Acid and base stress and transcriptomic responses in Bacillus subtilis.
Appl Environ Microbiol: 2009, 75(4);981-90
[PubMed:19114526] [WorldCat.org] [DOI] (I p)

Le Thi Tam, Christine Eymann, Haike Antelmann, Dirk Albrecht, Michael Hecker
Global gene expression profiling of Bacillus subtilis in response to ammonium and tryptophan starvation as revealed by transcriptome and proteome analysis.
J Mol Microbiol Biotechnol: 2007, 12(1-2);121-30
[PubMed:17183219] [WorldCat.org] [DOI] (P p)

Bauke Oudega, Micheline Vandenbol, Gregory Koningstein
A 12 kb nucleotide sequence containing the alanine dehydrogenase gene at 279 degrees on the Bacillus subtilis chromosome.
Microbiology (Reading): 1997, 143 ( Pt 5);1489-1491
[PubMed:9168598] [WorldCat.org] [DOI] (P p)

K J Siranosian, K Ireton, A D Grossman
Alanine dehydrogenase (ald) is required for normal sporulation in Bacillus subtilis.
J Bacteriol: 1993, 175(21);6789-96
[PubMed:8226620] [WorldCat.org] [DOI] (P p)

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