CwlC

• Description: N-acetylmuramoyl-L-alanine amidase
  
  

Gene name	cwlC
Synonyms
Essential	no
Product	N-acetylmuramoyl-L-alanine amidase
Function	mother cell lysis
MW, pI	27 kDa, 5.939
Gene length, protein length	765 bp, 255 aa
Immediate neighbours	ymaB, spoVK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

cell wall/ other, sporulation proteins

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

• Locus tag: BSU17410

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)

• Protein family: N-acetylmuramoyl-L-alanine amidase 3 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • secreted (according to Swiss-Prot)

Database entries

• Structure: 1X60 (peptidoglycan binding domain)

• UniProt: Q06320

• KEGG entry: [3]

• E.C. number: 3.5.1.28

Expression and regulation

• Operon: cwlC PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigK) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Masaki Mishima, Toshio Shida, Kazuto Yabuki, Ken-Ichi Kato, Junichi Sekiguchi, Chojiro Kojima
Solution structure of the peptidoglycan binding domain of Bacillus subtilis cell wall lytic enzyme CwlC: characterization of the sporulation-related repeats by NMR.
Biochemistry: 2005, 44(30);10153-63
[PubMed:16042392] [WorldCat.org] [DOI] (P p)

T Shida, H Hattori, F Ise, J Sekiguchi
Mutational analysis of catalytic sites of the cell wall lytic N-acetylmuramoyl-L-alanine amidases CwlC and CwlV.
J Biol Chem: 2001, 276(30);28140-6
[PubMed:11375403] [WorldCat.org] [DOI] (P p)

T Shida, H Hattori, F Ise, J Sekiguchi
Overexpression, purification, and characterization of Bacillus subtilis N-acetylmuramoyl-L-alanine amidase CwlC.
Biosci Biotechnol Biochem: 2000, 64(7);1522-5
[PubMed:10945275] [WorldCat.org] [DOI] (P p)

A Kuroda, Y Asami, J Sekiguchi
Molecular cloning of a sporulation-specific cell wall hydrolase gene of Bacillus subtilis.
J Bacteriol: 1993, 175(19);6260-8
[PubMed:8407798] [WorldCat.org] [DOI] (P p)