AppF

• Description: oligopeptide ABC transporter (ATP-binding protein)
  
  

Gene name	appF
Synonyms
Essential	no
Product	oligopeptide ABC transporter (ATP-binding protein))
Function	uptake of oligopeptides
Interactions involving this protein in SubtInteract: AppF
Metabolic function and regulation of this protein in SubtiPathways: Alternative nitrogen sources
MW, pI	36 kDa, 9.645
Gene length, protein length	987 bp, 329 aa
Immediate neighbours	appD, appA/1
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

ABC transporters, utilization of nitrogen sources other than amino acids, membrane proteins

This gene is a member of the following regulons

CodY regulon, ScoC regulon

The gene

Basic information

• Locus tag: BSU11370

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ABC transporter domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • (AppD-AppF)-(AppB-AppC)-AppA PubMed

• Localization: attached to the cell membrane (via AppB-AppC) PubMed

Database entries

• Structure:

• UniProt: P42065

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: appD-appF-appA-appB-appC PubMed

• Sigma factor:

• Regulation:
  • repressed during exponential growth (ScoC) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • ScoC: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: