PdaA

• Description: polysaccharide deacetylase, required for germination
  
  

Gene name	pdaA
Synonyms	yfjS
Essential	no
Product	polysaccharide deacetylase
Function	germination
MW, pI	29 kDa, 7.318
Gene length, protein length	789 bp, 263 aa
Immediate neighbours	yfjT, yfjR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

germination

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

• Locus tag: BSU07980

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: polysaccharide deacetylase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1W17

• UniProt: O34928

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: pdaA PubMed

• Sigma factor: SigG PubMed

• Regulation: expressed late during sporulation in the forespore (SigG) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Tatsuya Fukushima, Toshihiko Kitajima, Junichi Sekiguchi
A polysaccharide deacetylase homologue, PdaA, in Bacillus subtilis acts as an N-acetylmuramic acid deacetylase in vitro.
J Bacteriol: 2005, 187(4);1287-92
[PubMed:15687192] [WorldCat.org] [DOI] (P p)

David E Blair, Daan M F van Aalten
Structures of Bacillus subtilis PdaA, a family 4 carbohydrate esterase, and a complex with N-acetyl-glucosamine.
FEBS Lett: 2004, 570(1-3);13-9
[PubMed:15251431] [WorldCat.org] [DOI] (P p)

Meghan E Gilmore, Dabanjan Bandyopadhyay, Amanda M Dean, Sarah D Linnstaedt, David L Popham
Production of muramic delta-lactam in Bacillus subtilis spore peptidoglycan.
J Bacteriol: 2004, 186(1);80-9
[PubMed:14679227] [WorldCat.org] [DOI] (P p)

Tatsuya Fukushima, Hiroki Yamamoto, Abdelmadjid Atrih, Simon J Foster, Junichi Sekiguchi
A polysaccharide deacetylase gene (pdaA) is required for germination and for production of muramic delta-lactam residues in the spore cortex of Bacillus subtilis.
J Bacteriol: 2002, 184(21);6007-15
[PubMed:12374835] [WorldCat.org] [DOI] (P p)