pdaA

pdaA
168

N-acetylmuramic acid deacetylase, spore cortex peptidoglycan synthesis

Locus
BSU_07980
Molecular weight
29.92 kDa
Isoelectric point
7.32
Protein length
263 aaSequenceBlast
Gene length
792 bpSequenceBlast
Function
spore cortex peptidoglycan synthesis
Product
N-acetylmuramic acid deacetylase
Essential
no
Synonyms
pdaA, yfjS
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0726 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
869,559  870,350
Phenotypes of a mutant
improved UV resistance of spores at the cost of attenuated heat resistance and viable spore yield PubMed
The protein
Catalyzed reaction/ biological activity
de-N-acetylation of N-acetylglucosamine and N-acetyl-muramic acid for spore cortex peptidoglycan PubMed
Protein family
polysaccharide deacetylase family (according to UniProt)
Domains
NodB homology domain (aa 66-247) (according to UniProt)
Structure
1W17 (PDB) PubMed
O34928 (AlphaFold)
Expression and Regulation
Operons
Genes
pdaA
Description
PubMed
Regulation
expressed late during sporulation in the forespore (SigG) PubMed
Sigma factors
SigG: sigma factor, PubMed, in sigG regulon
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pdaA

2025-03-26 00:27:54

ghost

76

83ed6beb6bf99803edf51e8db78894ad277c8f43

324161C7FFAE730B620CD93E09F2D90F78052798

Biological materials
Mutant
MGNA-C273 (yfjS::erm), available at the NBRP B. subtilis, Japan
BKE07980 (pdaA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGCCAGCGCTCCTTCTG,  downstream forward: _UP4_TAAAAGAGAAAGACCTCTCC
BKK07980 (pdaA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGCCAGCGCTCCTTCTG,  downstream forward: _UP4_TAAAAGAGAAAGACCTCTCC
References
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Page visits: 4832

Time of last update: 2025-04-02 02:27:44

Author of last update: Jstuelk