MgsA

• Description: methylglyoxal synthase
  
  

Gene name	mgsA
Synonyms	ypjF, jojF
Essential	no
Product	methylglyoxal synthase
Function	bypass of glycolysis
Interactions involving this protein in SubtInteract: MgsA
Metabolic function and regulation of this protein in SubtiPathways: Lys, Thr, Central C-metabolism
MW, pI	14 kDa, 4.919
Gene length, protein length	411 bp, 137 aa
Immediate neighbours	bshB1, dapB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

carbon core metabolism

The gene

Basic information

• Locus tag: BSU22480

Expression

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Glycerone phosphate = methylglyoxal + phosphate (according to Swiss-Prot)

• Protein family: RNase Z family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • non-phosphorylated Crh interacts with MgsA to inhibit its activity PubMed

• Interactions:
  • Crh-MgsA (only non-phosphorylated Crh interacts with MgsA) PubMed

• Localization:

Database entries

• Structure: 1B93 (from Escherichia coli, 50% identity, 73% similarity) PubMed

• UniProt: P42980

• KEGG entry: [2]

• E.C. number: 4.2.3.3

Expression and regulation

• Operon: ypjD-dapB-mgsA-bshB1-bshA-cca-birA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • GP67 (mgsA::tet), available in Stülke lab
  • GP84 (mgsA::pX2(cat)), available in Stülke lab

• Expression vector:
  • pGP1301 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Stülke lab
  • pGP1180 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
  • pGP1181 (C-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP382), available in Stülke lab
  • pGP2207 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP1459), available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab

• FLAG-tag construct: GP86 (spc, based on pGP1331), available in the Stülke lab

• Antibody:

Labs working on this gene/protein

Boris Görke, University of Göttingen, Germany Homepage