Prs

• Description: phosphoribosylpyrophosphate synthetase, universally conserved protein
  
  

Gene name	prs
Synonyms
Essential	yes PubMed
Product	phosphoribosylpyrophosphate synthetase
Function	phosphoribosylpyrophosphate synthesis (biosynthesis of histidine)
Interactions involving this protein in SubtInteract: Prs
Metabolic function and regulation of this protein in SubtiPathways: Purine synthesis, Nucleotides (regulation), His, Murein recycling
MW, pI	34 kDa, 5.895
Gene length, protein length	951 bp, 317 aa
Immediate neighbours	gcaD, ctc
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, essential genes, universally conserved proteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU00510

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate (according to Swiss-Prot)

• Protein family: ribose-phosphate pyrophosphokinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • subject to feedback inhibition by two end products of purine biosynthesis, adenosine 5'-diphosphate (ADP) and guanosine 5'-diphosphate (GDP) PubMed

• Interactions: PurR-Prs

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1IBS

• UniProt: P14193

• KEGG entry: [3]

• E.C. number: 2.7.6.1

Additional information

universally conserved protein

Expression and regulation

• Operon: gcaD-prs-ctc PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed