CtaA

• Description: heme A synthase
  
  

Gene name	ctaA
Synonyms
Essential	no
Product	heme A synthase
Function	heme biosynthesis
MW, pI	33 kDa, 9.644
Gene length, protein length	918 bp, 306 aa
Immediate neighbours	pycA, ctaB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis of cofactors, sporulation proteins, membrane proteins

This gene is a member of the following regulons

ResD regulon, SigE regulon

The gene

Basic information

• Locus tag: BSU14870

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: COX15/ctaA family, Type 1 subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P12946

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ctaA PubMed

• Sigma factor: SigA PubMed, SigE PubMed

• Regulation:
  • expressed under anaerobic conditions (ResD) PubMed

• Regulatory mechanism:
  • ResD: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Tatsushi Mogi
Probing structure of heme A synthase from Bacillus subtilis by site-directed mutagenesis.
J Biochem: 2009, 145(5);625-33
[PubMed:19174544] [WorldCat.org] [DOI] (I p)

Anna Lewin, Lars Hederstedt
Promoted evolution of a shortened variant of heme A synthase in the membrane of Bacillus subtilis.
FEBS Lett: 2008, 582(9);1330-4
[PubMed:18358840] [WorldCat.org] [DOI] (P p)

Lars Hederstedt, Anna Lewin, Mimmi Throne-Holst
Heme A synthase enzyme functions dissected by mutagenesis of Bacillus subtilis CtaA.
J Bacteriol: 2005, 187(24);8361-9
[PubMed:16321940] [WorldCat.org] [DOI] (P p)

Matthew Schau, Amr Eldakak, F Marion Hulett
Terminal oxidases are essential to bypass the requirement for ResD for full Pho induction in Bacillus subtilis.
J Bacteriol: 2004, 186(24);8424-32
[PubMed:15576792] [WorldCat.org] [DOI] (P p)

Brienne M Brown, Zhihong Wang, Kenneth R Brown, Julia A Cricco, Eric L Hegg
Heme O synthase and heme A synthase from Bacillus subtilis and Rhodobacter sphaeroides interact in Escherichia coli.
Biochemistry: 2004, 43(42);13541-8
[PubMed:15491161] [WorldCat.org] [DOI] (P p)

S Paul, X Zhang, F M Hulett
Two ResD-controlled promoters regulate ctaA expression in Bacillus subtilis.
J Bacteriol: 2001, 183(10);3237-46
[PubMed:11325953] [WorldCat.org] [DOI] (P p)

X Zhang, F M Hulett
ResD signal transduction regulator of aerobic respiration in Bacillus subtilis: ctaA promoter regulation.
Mol Microbiol: 2000, 37(5);1208-19
[PubMed:10972837] [WorldCat.org] [DOI] (P p)

B Svensson, K K Andersson, L Hederstedt
Low-spin heme A in the heme A biosynthetic protein CtaA from Bacillus subtilis.
Eur J Biochem: 1996, 238(1);287-95
[PubMed:8665949] [WorldCat.org] [DOI] (P p)

B Svensson, L Hederstedt
Bacillus subtilis CtaA is a heme-containing membrane protein involved in heme A biosynthesis.
J Bacteriol: 1994, 176(21);6663-71
[PubMed:7961419] [WorldCat.org] [DOI] (P p)

B Svensson, M Lübben, L Hederstedt
Bacillus subtilis CtaA and CtaB function in haem A biosynthesis.
Mol Microbiol: 1993, 10(1);193-201
[PubMed:7968515] [WorldCat.org] [DOI] (P p)

J P Mueller, H W Taber
Structure and expression of the cytochrome aa3 regulatory gene ctaA of Bacillus subtilis.
J Bacteriol: 1989, 171(9);4979-86
[PubMed:2549007] [WorldCat.org] [DOI] (P p)