Difference between revisions of "PdhB"
(→References) |
(→Expression and regulation) |
||
| Line 91: | Line 91: | ||
* '''Operon:''' ''[[pdhA]]-[[pdhB]]-[[pdhC]]-[[pdhD]]'' | * '''Operon:''' ''[[pdhA]]-[[pdhB]]-[[pdhC]]-[[pdhD]]'' | ||
| − | * '''Sigma factor:''' | + | * '''Sigma factor:''' [[SigA]] |
| − | * '''Regulation:''' | + | * '''Regulation:''' weak induction by glucose [http://www.ncbi.nlm.nih.gov/sites/entrez/12850135 PubMed] |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
Revision as of 14:58, 9 February 2009
- Description: pyruvate dehydrogenase (E1 beta subunit)
| Gene name | pdhB |
| Synonyms | |
| Essential | no |
| Product | pyruvate dehydrogenase (E1 beta subunit) |
| Function | links glycolysis and TCA cycle |
| MW, pI | 35 kDa, 4.547 |
| Gene length, protein length | 975 bp, 325 aa |
| Immediate neighbours | pdhA, pdhC |
| Gene sequence (+200bp) | Protein sequence |
Genetic context 
| |
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
defects in sporulation and unable to grow on glucose as single carbon source PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: phosphorylated on Ser-302 or Ser-306 PubMed
- Cofactor(s):
- Effectors of protein activity:
- Localization: membrane associated PubMed
Database entries
- Structure: 1W88 (E1 in complex with subunit binding domain of E2, Geobacillus stearothermophilus)
- Swiss prot entry: [3]
- KEGG entry:
- E.C. number: 1.2.4.1
Additional information
Expression and regulation
- Sigma factor: SigA
- Regulation: weak induction by glucose PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
- Macek et al. (2007) The serine/ threonine/ tyrosine phosphoproteome of the model bacterium Bacillus subtilis. Mol. Cell. Proteomics 6: 697-707 PubMed
- Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing Bacillus subtilis by divergent approaches. Proteomics 8: 4123-4136 PubMed
- Gao et al. (2002) The E1beta and E2 subunits of the Bacillus subtilis pyruvate dehydrogenase complex are involved in regulation of sporulation.J. Bacteriol. 184: 2780-2788. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
