Xis

• Description: excisionase
  
  

Gene name	xis
Synonyms	ydzC, sacV
Essential	no
Product	excisionase
Function	excision of the conjugative transposon ICEBs1 from the trnS-leu2 locus
MW, pI	7 kDa, 9.64
Gene length, protein length	192 bp, 64 aa
Immediate neighbours	immR, ydcO
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU04830

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

mobile genetic elements

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: excision of conjugative transposon PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O31490

• KEGG entry: [2]

• E.C. number:

Additional information

Was reported to be involved in the regulation of the levansucrase gene (sacB)

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • ImmR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Catherine A Lee, Jennifer M Auchtung, Rita E Monson, Alan D Grossman
Identification and characterization of int (integrase), xis (excisionase) and chromosomal attachment sites of the integrative and conjugative element ICEBs1 of Bacillus subtilis.
Mol Microbiol: 2007, 66(6);1356-69
[PubMed:18005101] [WorldCat.org] [DOI] (P p)

Jennifer M Auchtung, Catherine A Lee, Katherine L Garrison, Alan D Grossman
Identification and characterization of the immunity repressor (ImmR) that controls the mobile genetic element ICEBs1 of Bacillus subtilis.
Mol Microbiol: 2007, 64(6);1515-28
[PubMed:17511812] [WorldCat.org] [DOI] (P p)