SacX

• Description: trigger enzyme: sucrose-specific phosphotransferase system, EIIBC component of the PTS (low affinity) and control of SacY activity
  
  

Gene name	sacX
Synonyms	ipa-14r, sacS
Essential	no
Product	trigger enzyme: sucrose-specific phosphotransferase system, EIIBC component (low affinity)
Function	sucrose uptake and phosphorylation, control of SacY activity
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	48 kDa, 9.346
Gene length, protein length	1377 bp, 459 aa
Immediate neighbours	epr, sacY
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU38410

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

phosphotransferase systems, utilization of specific carbon sources, trigger enzyme, membrane proteins, phosphoproteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)

• Protein family: PTS permease, sucrose permease (Scr) family PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P15400

• KEGG entry: [3]

• E.C. number: 2.7.1.69

Expression and regulation

• Operon: sacX-sacY PubMed

• Sigma factor: SigA PubMed

• Regulation: induction by sucrose (at high concentration) PubMed

• Regulatory mechanism:
  • induction by SacY-dependent RNA switch (transcriptional antitermination) PubMed
  • DegU-P: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: