PurT

• Description: phosphoribosylglycinamide formyltransferase 2
  
  

Gene name	purT
Synonyms
Essential	no
Product	phosphoribosylglycinamide formyltransferase 2
Function	purine biosynthesis
Metabolic function and regulation of this protein in SubtiPathways: Purine synthesis, Nucleotides (regulation)
MW, pI	41 kDa, 4.722
Gene length, protein length	1152 bp, 384 aa
Immediate neighbours	ybfI, mpr
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU02230

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Formate + ATP + 5'-phospho-ribosylglycinamide = 5'-phosphoribosyl-N-formylglycinamide + ADP + diphosphate (according to Swiss-Prot)

• Protein family: ATP-grasp domain (according to Swiss-Prot)

• Paralogous protein(s): PurK

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1KJ9 (with Mg-ATP from Escherichia coli, 55% identity, 69% similarity) PubMed

• UniProt: P39771

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: purT (according to DBTBS)

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

H H Saxild, J H Jacobsen, P Nygaard
Functional analysis of the Bacillus subtilis purT gene encoding formate-dependent glycinamide ribonucleotide transformylase.
Microbiology (Reading): 1995, 141 ( Pt 9);2211-8
[PubMed:7496533] [WorldCat.org] [DOI] (P p)