YvcL

• Description: WhiA family protein conserved and ubiquitous among gram-positive bacteria, putative transcription factor
  
  

Gene name	yvcL
Synonyms
Essential	no
Product	unknown
Function	unknown
MW, pI	36 kDa, 9.479
Gene length, protein length	948 bp, 316 aa
Immediate neighbours	crh, yvcK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU34750

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: WhiA family PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: suggested HTH domain PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3HYI (Crystal structure of full-length DUF199/WhiA from Thermatoga maritima), 3HYJ (Crystal structure of the N-terminal LAGLIDADG domain of DUF199/WhiA)

• UniProt: O06975

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yvcI-yvcJ-yvcK-yvcL-crh-yvcN PubMed

• Sigma factor: SigA PubMed

• Regulation: very weak stimuation of expression by citrate and succinate PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP231 (amyE::PrpsJ-lacZ, yvcL::cat) available in Stülke lab; plasmid pGP1468 was used to disrupt the gene.

• Expression vector: pGP1469 for expression in B. subtilis (in pGP382, with C-terminal STREP-TAG)

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab

• Antibody:

Your additional remarks

WhiA orthologues are present in all Gram-positive bacteria and best characterized by the WhiA sporulation control factor in Streptomyces coelicolor. They are thought to act as genetic regulators that have evolved from LAGLIDADG homing endonucleases (LHE).