SrtN

• Description: protein deacetylase for the control of AcsA activity
  
  

Gene name	srtN
Synonyms	yhdZ
Essential	no
Product	NAD(+)-dependent AcsA deacetylase
Function	control of AcsA activity
MW, pI	27 kDa, 5.134
Gene length, protein length	741 bp, 247 aa
Immediate neighbours	yhdY, yheN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU09650

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: NAD⁺ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein (according to Swiss-Prot) deacetylates (and thereby activates) AcsA (together with AcuC) PubMed

• Protein family: deacetylase sirtuin-type domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O07595

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • GP1210 (srtN::cat), available in Stülke lab
  • GP1213 (srtN::cat)(acuC::kan), available in Stülke lab
• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jeffrey G Gardner, Jorge C Escalante-Semerena
In Bacillus subtilis, the sirtuin protein deacetylase, encoded by the srtN gene (formerly yhdZ), and functions encoded by the acuABC genes control the activity of acetyl coenzyme A synthetase.
J Bacteriol: 2009, 191(6);1749-55
[PubMed:19136592] [WorldCat.org] [DOI] (I p)