Difference between revisions of "MaeA"
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=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' | + | * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=1WW8 1WW8] (from ''Pyrococcus horikoshii'', 46% identity, 63% similarity) |
* '''UniProt:''' [http://www.uniprot.org/uniprot/P45868 P45868] | * '''UniProt:''' [http://www.uniprot.org/uniprot/P45868 P45868] | ||
Revision as of 10:49, 21 July 2010
- Description: malic enzyme
| Gene name | maeA |
| Synonyms | ywkA |
| Essential | no |
| Product | NAD-dependent malate dehydrogenase |
| Function | malate utilization |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
| MW, pI | 63 kDa, 4.883 |
| Gene length, protein length | 1746 bp, 582 aa |
| Immediate neighbours | ywkB, tdk |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU37050
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (S)-malate + NAD+ = pyruvate + CO2 + NADH (according to Swiss-Prot) malate --> pyruvate
- Protein family: malic enzymes family (according to Swiss-Prot)
- Paralogous protein(s): MalS
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure: 1WW8 (from Pyrococcus horikoshii, 46% identity, 63% similarity)
- UniProt: P45868
- KEGG entry: [3]
- E.C. number: 1.1.1.38
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
Your additional remarks
References
Thierry Doan, Pascale Servant, Shigeo Tojo, Hirotake Yamaguchi, Guillaume Lerondel, Ken-Ichi Yoshida, Yasutaro Fujita, Stéphane Aymerich
The Bacillus subtilis ywkA gene encodes a malic enzyme and its transcription is activated by the YufL/YufM two-component system in response to malate.
Microbiology (Reading): 2003, 149(Pt 9);2331-2343
[PubMed:12949160]
[WorldCat.org]
[DOI]
(P p)
