Ald

• Description: L-alanine dehydrogenase
  
  

Gene name	ald
Synonyms	spoVN
Essential	no
Product	L-alanine dehydrogenase
Function	alanine utilization
Metabolic function and regulation of this protein in SubtiPathways: Ala, Gly, Ser
MW, pI	39 kDa, 5.125
Gene length, protein length	1134 bp, 378 aa
Immediate neighbours	yukF, yukJ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU31930

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-alanine + H₂O + NAD⁺ = pyruvate + NH₃ + NADH (according to Swiss-Prot)

• Protein family: AlaDH/PNT family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2EEZ (from Thermus thermophilus hb8 mutant, 55% identity, 66% similarity)

• UniProt: Q08352

• KEGG entry: [3]

• E.C. number: 1.4.1.1

Expression and regulation

• Operon: ald PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by alanine PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: