Dra

• Description: deoxyribose-phosphate aldolase
  
  

Gene name	dra
Synonyms
Essential	no
Product	deoxyribose-phosphate aldolase
Function	utilization of nucleotides as carbon source
Metabolic function and regulation of this protein in SubtiPathways: Nucleoside catabolism, Nucleotides (regulation)
MW, pI	22 kDa, 4.926
Gene length, protein length	633 bp, 211 aa
Immediate neighbours	nupC, deoR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU39420

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2-deoxy-D-ribose 5-phosphate = D-glyceraldehyde 3-phosphate + acetaldehyde (according to Swiss-Prot)

• Protein family: DeoC type 1 subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1MZH (from Aquifex aeolicus, 42% identity, 62% similarity)

• UniProt: P39121

• KEGG entry: [3]

• E.C. number: 4.1.2.4

Expression and regulation

• Operon: dra-nupC-pdp PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed
  • induced by deoxynucleotides (DeoR) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • DeoR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Xianmin Zeng, Anne Galinier, Hans H Saxild
Catabolite repression of dra-nupC-pdp operon expression in Bacillus subtilis.
Microbiology (Reading): 2000, 146 ( Pt 11);2901-2908
[PubMed:11065368] [WorldCat.org] [DOI] (P p)

Y Miwa, A Nakata, A Ogiwara, M Yamamoto, Y Fujita
Evaluation and characterization of catabolite-responsive elements (cre) of Bacillus subtilis.
Nucleic Acids Res: 2000, 28(5);1206-10
[PubMed:10666464] [WorldCat.org] [DOI] (I p)

X Zeng, H H Saxild
Identification and characterization of a DeoR-specific operator sequence essential for induction of dra-nupC-pdp operon expression in Bacillus subtilis.
J Bacteriol: 1999, 181(6);1719-27
[PubMed:10074062] [WorldCat.org] [DOI] (P p)

H H Saxild, L N Andersen, K Hammer
Dra-nupC-pdp operon of Bacillus subtilis: nucleotide sequence, induction by deoxyribonucleosides, and transcriptional regulation by the deoR-encoded DeoR repressor protein.
J Bacteriol: 1996, 178(2);424-34
[PubMed:8550462] [WorldCat.org] [DOI] (P p)