NasD

• Description: assimilatory nitrite reductase (subunit)
  
  

Gene name	nasD
Synonyms	nasBC, nirB
Essential	no
Product	assimilatory nitrite reductase (subunit)
Function	utilization of nitrite as nitrogen source
MW, pI	88 kDa, 4.948
Gene length, protein length	2415 bp, 805 aa
Immediate neighbours	nasE, nasC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU03300

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Ammonium hydroxide + 3 NAD(P)⁺ + H₂O = nitrite + 3 NAD(P)H (according to Swiss-Prot)

• Protein family: nitrite and sulfite reductase 4Fe-4S domain family (according to Swiss-Prot)

• Paralogous protein(s): NasB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: NasD-NasE

• Localization:

Database entries

• Structure:

• UniProt: P42435

• KEGG entry: [3]

• E.C. number: 1.7.1.4

Expression and regulation

• Operon: nasB-nasC-nasD-nasE-nasF PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • expressed under anaerobic conditions (ResD) PubMed
  • repressed unless NO is present under anaerobic conditions (NsrR) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed
  • ResD: transcription activation PubMed
  • NsrR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: