NasC

• Description: nitrate reductase (catalytic subunit)
  
  

Gene name	nasC
Synonyms	narB, nasBB
Essential	no
Product	nitrate reductase (catalytic subunit)
Function	nitrate respiration
MW, pI	78 kDa, 6.728
Gene length, protein length	2130 bp, 710 aa
Immediate neighbours	nasD, nasB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU03310

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Nitrite + acceptor = nitrate + reduced acceptor (according to Swiss-Prot)

• Protein family: prokaryotic molybdopterin-containing oxidoreductase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: NasB-NasC

• Localization:

Database entries

• Structure:

• UniProt: P42434

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: nasB-nasC-nasD-nasE-nasF PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • repressed unless NO is present under anaerobic conditions (NsrR) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed
  • NsrR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Michiko M Nakano, Hao Geng, Shunji Nakano, Kazuo Kobayashi
The nitric oxide-responsive regulator NsrR controls ResDE-dependent gene expression.
J Bacteriol: 2006, 188(16);5878-87
[PubMed:16885456] [WorldCat.org] [DOI] (P p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

L V Wray, A E Ferson, K Rohrer, S H Fisher
TnrA, a transcription factor required for global nitrogen regulation in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1996, 93(17);8841-5
[PubMed:8799114] [WorldCat.org] [DOI] (P p)