Difference between revisions of "PucH"
| Line 90: | Line 90: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' ''pucH'' {{PubMed|12029039}} |
| − | * '''[[Sigma factor]]:''' | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|12029039}} |
* '''Regulation:''' | * '''Regulation:''' | ||
| − | ** induced in the presence of purine nucleotides (inducer: allantoin) ([[PucR]]) | + | ** induced in the presence of purine nucleotides (inducer: allantoin) ([[PucR]]) {{PubMed|12029039}} |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| − | ** [PucR]]: transcription activation | + | ** [[PucR]]: transcription activation {{PubMed|12029039}} |
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 122: | Line 122: | ||
=References= | =References= | ||
| − | <pubmed>11344136, | + | <pubmed>11344136,,12029039, </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 15:04, 28 December 2009
- Description: allantoinase
| Gene name | pucH |
| Synonyms | yunH |
| Essential | no |
| Product | allantoinase |
| Function | purine utilization |
| Metabolic function and regulation of this protein in SubtiPathways: Purine catabolism, Nucleotides (regulation) | |
| MW, pI | 48 kDa, 5.062 |
| Gene length, protein length | 1338 bp, 446 aa |
| Immediate neighbours | yunG, pucR |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU32410
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (S)-allantoin + H2O = allantoate (according to Swiss-Prot)
- Protein family: Allantoinase subfamily (according to Swiss-Prot)
- Paralogous protein(s): PyrC
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: O32137
- KEGG entry: [3]
- E.C. number: 3.5.2.5
Additional information
Expression and regulation
- Operon: pucH PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Lars Beier, Per Nygaard, Hanne Jarmer, Hans H Saxild
Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism.
J Bacteriol: 2002, 184(12);3232-41
[PubMed:12029039]
[WorldCat.org]
[DOI]
(P p)
A C Schultz, P Nygaard, H H Saxild
Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.
J Bacteriol: 2001, 183(11);3293-302
[PubMed:11344136]
[WorldCat.org]
[DOI]
(P p)
