AbbA

• Description: inhibitor of AbrB activity
  
  

Gene name	abbA
Synonyms	ykzF
Essential	no
Product	anti-repressor
Function	inhibition of AbrB
MW, pI	7 kDa, 4.642
Gene length, protein length	195 bp, 65 aa
Immediate neighbours	ykuK, ykuL
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU14120

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: inhibition of AbrB activity PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: AbbA-AbrB PubMed

• Localization:

Database entries

• Structure:

• UniProt: O31697

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ykuJ-ykuK-abbA-ykuL-ccpC PubMed

• Sigma factor:

• Regulation:
  • repressed by glucose (4.3-fold) (CcpA) PubMed
  • expressed at onset of stationary phase (Spo0A) PubMed
  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • Spo0A: transcription activation by Spo0A~P PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion: pGP708 (in pAC5), available in Stülke lab, pGP712 (in pAC6), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Richard Losick, Harvard Univ., Cambridge, USA homepage

References

Allison V Banse, Arnaud Chastanet, Lilah Rahn-Lee, Errett C Hobbs, Richard Losick
Parallel pathways of repression and antirepression governing the transition to stationary phase in Bacillus subtilis.
Proc Natl Acad Sci U S A: 2008, 105(40);15547-52
[PubMed:18840696] [WorldCat.org] [DOI] (I p)

Hans-Matti Blencke, Irene Reif, Fabian M Commichau, Christian Detsch, Ingrid Wacker, Holger Ludwig, Jörg Stülke
Regulation of citB expression in Bacillus subtilis: integration of multiple metabolic signals in the citrate pool and by the general nitrogen regulatory system.
Arch Microbiol: 2006, 185(2);136-46
[PubMed:16395550] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)