NrgB

• Description: nitrogen-regulated PII-like protein
  
  

Gene name	nrgB
Synonyms	glnK
Essential	no
Product	nitrogen-regulated PII-like protein
Function	regulation of ammonium uptake
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	12 kDa, 8.825
Gene length, protein length	348 bp, 116 aa
Immediate neighbours	nrgA, bcrC
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU36520

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: P(II) protein family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: NrgA-NrgB PubMed, TnrA-NrgB PubMed

• Localization: cell membrane (according to Swiss-Prot), membrane

Database entries

• Structure:

• UniProt: Q07428

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: nrgA-nrgB

• Sigma factor: SigA

• Regulation: induced by ammonium limitation
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

• Regulatory mechanism: transcription activation by TnrA
  • TnrA: transcription activation PubMed

• Additional information:

Biological materials

• Mutant: GP259 (cat), GP255 (nrgAB, cat), both available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: available in Jörg Stülke lab

Labs working on this gene/protein

Susan Fisher, Boston, USA homepage

References

Annette Heinrich, Kathrin Woyda, Katja Brauburger, Gregor Meiss, Christian Detsch, Jörg Stülke, Karl Forchhammer
Interaction of the membrane-bound GlnK-AmtB complex with the master regulator of nitrogen metabolism TnrA in Bacillus subtilis.
J Biol Chem: 2006, 281(46);34909-17
[PubMed:17001076] [WorldCat.org] [DOI] (P p)

Christian Detsch, Jörg Stülke
Ammonium utilization in Bacillus subtilis: transport and regulatory functions of NrgA and NrgB.
Microbiology (Reading): 2003, 149(Pt 11);3289-3297
[PubMed:14600241] [WorldCat.org] [DOI] (P p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

L V Wray, A E Ferson, K Rohrer, S H Fisher
TnrA, a transcription factor required for global nitrogen regulation in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1996, 93(17);8841-5
[PubMed:8799114] [WorldCat.org] [DOI] (P p)

L V Wray, M R Atkinson, S H Fisher
The nitrogen-regulated Bacillus subtilis nrgAB operon encodes a membrane protein and a protein highly similar to the Escherichia coli glnB-encoded PII protein.
J Bacteriol: 1994, 176(1);108-14
[PubMed:8282685] [WorldCat.org] [DOI] (P p)

M R Atkinson, S H Fisher
Identification of genes and gene products whose expression is activated during nitrogen-limited growth in Bacillus subtilis.
J Bacteriol: 1991, 173(1);23-7
[PubMed:1670935] [WorldCat.org] [DOI] (P p)