Difference between revisions of "RpsJ"

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(References)
(Biological materials)
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* '''Expression vector:'''
 
* '''Expression vector:'''
 
          
 
          
* '''lacZ fusion:'''
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* '''lacZ fusion:''' pGP605 (in [[pAC6]]), available  in [[Stülke]] lab
  
 
* '''GFP fusion:'''
 
* '''GFP fusion:'''

Revision as of 15:39, 2 September 2009

  • Description: ribosomal protein

Gene name rpsJ
Synonyms tetA
Essential yes PubMed
Product ribosomal protein S10 (BS13)
Function translation
MW, pI 11 kDa, 10.207
Gene length, protein length 306 bp, 102 aa
Immediate neighbours ybaC, rplC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RpsJ context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU01150

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: ribosomal protein S10P family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion: pGP605 (in pAC6), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Matthew A Lauber, William E Running, James P Reilly
B. subtilis ribosomal proteins: structural homology and post-translational modifications.
J Proteome Res: 2009, 8(9);4193-206
[PubMed:19653700] [WorldCat.org] [DOI] (P p)