AbrB

• Description: transcriptional regulator of transition state genes
  
  

Gene name	abrB
Synonyms	cpsX, tolB
Essential	no
Product	transcriptional regulator
Function	regulation of gene expression during the transition from growth to stationary phase
Metabolic function and regulation of this protein in SubtiPathways: His, Asp, Asn, Ammonium/ glutamate, Central C-metabolism, Sugar catabolism
MW, pI	10 kDa, 6.57
Gene length, protein length	282 bp, 94 aa
Immediate neighbours	yabC, metS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU00370

Phenotypes of a mutant

No swarming motility on B medium. PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): Abh, SpoVT (only N-terminal domain)

Genes/ operons controlled by AbrB

• Activated by AbrB: citB, comK, hpr, rbsR-rbsK-rbsD-rbsA-rbsC-rbsB

• Repressed by AbrB: abrB, aprE, ftsA-ftsZ, kinC, motA, nprE, pbpE, spo0H, spoVG, spo0E, tycA, sbo-alb, yqxM-sipW-tasA, sdpA operon, sunA operon, skfA operon, abh, pbpE, ggt, sinI-sinR, sigW, yuaB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: interaction with AbbA results in inactivation of AbrB PubMed

• Interactions: AbrB-AbbA PubMed

• Localization:

Database entries

• Structure: 1Z0R (N-terminal DNA recognition domain), 1Z0R (N-terminal DNA recognition domain) NCBI PubMed

• UniProt: P08874

• KEGG entry: [3]

Expression and regulation

• Operon: abrB PubMed

• Sigma factor: SigA PubMed

• Regulation: expressed at the onset of stationary phase PubMed
  • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • repressed during logrithmic growth (AbrB) PubMed

• Regulatory mechanism: repressed by Spo0A-P PubMed
  • Spo0A: transcription repression PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: TMB082 (aphA3), available in Thorsten Mascher lab, TT731 (aphA3)

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Richard Losick, Harvard Univ., Cambridge, USA homepage

Mark Strauch, Baltimore, USA homepage