PurS

• Description: phosphoribosylformylglycinamidine synthase
  
  

Gene name	purS
Synonyms	yexA
Essential	no
Product	phosphoribosylformylglycinamidine synthase
Function	purine biosynthesis
Metabolic function and regulation of this protein in SubtiPathways: Purine synthesis, Nucleotides (regulation), Stress
MW, pI	9 kDa, 4.594
Gene length, protein length	252 bp, 84 aa
Immediate neighbours	purC, purQ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU06460

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: UPF0062 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: PurS-PurS PubMed, PurS-PurQ-PurL PubMed

• Localization:

Database entries

• Structure: 1T4A

• UniProt: P12049

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: purE-purK-purB-purC-purS-purQ-purL-purF-purM-purN-purH-purD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of purine nucleotides (PurR) PubMed
  • repressed in the presence of adenine or adenosine(PurR) PubMed
  • repressed in the presence of guanine(G-box) PubMed

• Regulatory mechanism:
  • [PurR]]: transcription repression PubMed
  • PurR: transcription repression (molecular inducer: PRPP) PubMed
  • G-box: transcription termination/ antitermination (riboswitch) PubMed1 PubMed2

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ruchi Anand, Aaron A Hoskins, Eric M Bennett, Michael D Sintchak, JoAnne Stubbe, Steven E Ealick
A model for the Bacillus subtilis formylglycinamide ribonucleotide amidotransferase multiprotein complex.
Biochemistry: 2004, 43(32);10343-52
[PubMed:15301532] [WorldCat.org] [DOI] (P p)

Aaron A Hoskins, Ruchi Anand, Steven E Ealick, JoAnne Stubbe
The formylglycinamide ribonucleotide amidotransferase complex from Bacillus subtilis: metabolite-mediated complex formation.
Biochemistry: 2004, 43(32);10314-27
[PubMed:15301530] [WorldCat.org] [DOI] (P p)

Hans H Saxild, Per Nygaard
The yexA gene product is required for phosphoribosylformylglycinamidine synthetase activity in Bacillus subtilis.
Microbiology (Reading): 2000, 146 ( Pt 4);807-814
[PubMed:10784038] [WorldCat.org] [DOI] (P p)

M Weng, P L Nagy, H Zalkin
Identification of the Bacillus subtilis pur operon repressor.
Proc Natl Acad Sci U S A: 1995, 92(16);7455-9
[PubMed:7638212] [WorldCat.org] [DOI] (P p)

D J Ebbole, H Zalkin
Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis.
J Biol Chem: 1987, 262(17);8274-87
[PubMed:3036807] [WorldCat.org] (P p)