ArgJ

• Description: N-acetylglutamate synthase
  
  

Gene name	argJ
Synonyms	argA, argE
Essential	no
Product	N-acetylglutamate synthase
Function	biosynthesis of arginine
MW, pI	43 kDa, 4.703
Gene length, protein length	1218 bp, 406 aa
Immediate neighbours	argC, argB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU11200

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate (according to Swiss-Prot)

• Protein family: argJ family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: P36843

• KEGG entry: [3]

• E.C. number: 2.3.1.35

Expression and regulation

• Operon: argC-argJ-argB-argD-carA-carB-argF

• Sigma factor: SigA

• Regulation: repressed by casamino acids PubMed, repressed by arginine (AhrC)

• Regulatory mechanism: AhrC: transcription repression

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

A Mountain, N H Mann, R N Munton, S Baumberg
Cloning of a Bacillus subtilis restriction fragment complementing auxotrophic mutants of eight Escherichia coli genes of arginine biosynthesis.
Mol Gen Genet: 1984, 197(1);82-9
[PubMed:6096675] [WorldCat.org] [DOI] (P p)

1. Mäder et al. (2002) Transcriptome and Proteome Analysis of Bacillus subtilis Gene Expression Modulated by Amino Acid Availability. J. Bacteriol 184: 1844288-4295 PubMed
2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed