MtnX

• Description: 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase
  
  

Gene name	mtnX
Synonyms	ykrX
Essential	no
Product	2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase
Function	methionine salvage
MW, pI	26 kDa, 4.658
Gene length, protein length	705 bp, 235 aa
Immediate neighbours	mtnW, mtnB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU13600

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate + H₂O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate (according to Swiss-Prot)

• Protein family: MtnX family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2FEA

• Swiss prot entry: O31667

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mtnW-mtnX-mtnB-mtnD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed
  • induced by methionine starvation (S-box) PubMed

• Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Mäder et al. (2002) Transcriptome and Proteome Analysis of Bacillus subtilis Gene Expression Modulated by Amino Acid Availability. J. Bacteriol 184: 1844288-4295 PubMed
2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed