Difference between revisions of "CarB"
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* '''[[Sigma factor]]:''' [[SigA]] | * '''[[Sigma factor]]:''' [[SigA]] | ||
| − | * '''Regulation:''' repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] | + | * '''Regulation:''' repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed], repressed by arginine ([[ArgR]]) |
| − | * '''Regulatory mechanism:''' | + | * '''Regulatory mechanism:''' [[ArgR]]: transcription repression |
* '''Additional information:''' subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed] | * '''Additional information:''' subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed] | ||
Revision as of 04:51, 28 May 2009
- Description: carbamoyl-phosphate transferase-arginine subunit B)
| Gene name | carB |
| Synonyms | cpa |
| Essential | no |
| Product | carbamoyl-phosphate transferase-arginine subunit B) |
| Function | biosynthesis of arginine |
| MW, pI | 112 kDa, 4.712 |
| Gene length, protein length | 3090 bp, 1030 aa |
| Immediate neighbours | carA, argF |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate (according to Swiss-Prot)
- Protein family: carB family (according to Swiss-Prot)
- Paralogous protein(s): PyrAB
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- Swiss prot entry: P18185
- KEGG entry: BSU11240
- E.C. number: 6.3.5.5
Additional information
- subject to Clp-dependent proteolysis upon glucose starvation PubMed
Expression and regulation
- Regulatory mechanism: ArgR: transcription repression
- Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
- Mäder et al. (2002) Transcriptome and Proteome Analysis of Bacillus subtilis Gene Expression Modulated by Amino Acid Availability. J. Bacteriol 184: 1844288-4295 PubMed
- Gerth et al. (2008) Clp-dependent proteolysis down-regulates central metabolic pathways in glucose-starved Bacillus subtilis. J Bacteriol 190:321-331 PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
