Difference between revisions of "PurR"
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=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * ''' | + | * '''Biological activity:''' transcriptional repressor of purine biosynthetic operons |
* '''Protein family:''' | * '''Protein family:''' | ||
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* '''Cofactor(s):''' | * '''Cofactor(s):''' | ||
| − | * '''Effectors of protein activity:''' PRPP acts as inducer, binding results in release of PurR from the operator | + | * '''Effectors of protein activity:''' PRPP acts as inducer, binding results in release of PurR from the operator [http://www.ncbi.nlm.nih.gov/sites/entrez/10919400 PubMed] |
| − | * '''Interactions:''' | + | * '''Interactions:''' |
* '''Localization:''' | * '''Localization:''' | ||
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=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' 1P4A (complex with PRPP) [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?uid=25670 NCBI] | + | * '''Structure:''' 1P4A (complex with PRPP) [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?uid=25670 NCBI] [http://www.ncbi.nlm.nih.gov/sites/entrez/12837783 PubMed] |
* '''Swiss prot entry:''' | * '''Swiss prot entry:''' | ||
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' ''[[purR]]-[[yabJ]]'' | + | * '''Operon:''' ''[[purR]]-[[yabJ]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/7638212 PubMed] |
* '''Sigma factor:''' | * '''Sigma factor:''' | ||
| − | * '''Regulation:''' | + | * '''Regulation:''' negative autoregulation [http://www.ncbi.nlm.nih.gov/sites/entrez/7638212 PubMed] |
| − | * '''Regulatory mechanism:''' | + | * '''Regulatory mechanism:''' repression by [[PurR]] [http://www.ncbi.nlm.nih.gov/sites/entrez/7638212 PubMed] |
* '''Additional information:''' | * '''Additional information:''' | ||
Revision as of 14:28, 1 February 2009
- Description: purine repressor
| Gene name | purR |
| Synonyms | yabI |
| Essential | |
| Product | transcriptional regulator |
| Function | negative regulation of the purine operons ( purEKBCLQFMNHD, purA) |
| MW, pI | 31 kDa, 5.771 |
| Gene length, protein length | 855 bp, 285 aa |
| Immediate neighbours | |
| Gene sequence (+200bp) | Protein sequence |
Genetic context 
| |
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Biological activity: transcriptional repressor of purine biosynthetic operons
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity: PRPP acts as inducer, binding results in release of PurR from the operator PubMed
- Interactions:
- Localization:
Database entries
- Swiss prot entry:
- KEGG entry:
- E.C. number:
Additional information
requires YabJ for in vivo activity
Expression and regulation
- Sigma factor:
- Regulation: negative autoregulation PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
- Weng et al. (1995) Identification of the Bacillus subtilis pur operon repressor. Proc. Natl. Acad. Sci. USA 92: 7455-7459. PubMed
- Weng & Zalkin (2000) Mutations in the Bacillus subtilis purine repressor that perturb PRPP effector function in vitro and in vivo.Currr. Microbiol. 41: 56-59. PubMed
- Sinha et al. (2003) The purine repressor of Bacillus subtilis: a novel combination of domains adapted for transcription regulation.J. Bacteriol. 185: 4087-4098. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
