Difference between revisions of "Sandbox"

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* '''Description:''' protection against daptamycin <br/><br/>
+
* '''Description:''' negative effector of LiaR <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''liaH''
+
|''liaF''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yvqH''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yvqF''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
+
|style="background:#ABCDEF;" align="center"| '''Product''' || negative effector of LiaR
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || protection against daptamycin
+
|style="background:#ABCDEF;" align="center"|'''Function''' || control of LiaR activity
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 25 kDa, 6.172  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 26 kDa, 9.211  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 675 bp, 225 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 723 bp, 241 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[liaG]]'', ''[[liaI]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[liaS]]'', ''[[liaG]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Hier soll was neues rein'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:yvqH_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:yvqF_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 43: Line 43:
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yvqIHGFEC.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yvqIHGFEC.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG14137]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG14135]
  
 
=== Additional information===
 
=== Additional information===
Line 80: Line 80:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU33120]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU33100]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 90: Line 90:
 
* '''Operon:''' ''[[liaI]]-[[liaH]]-[[liaG]]-[[liaF]]-[[liaS]]-[[liaR]]''  
 
* '''Operon:''' ''[[liaI]]-[[liaH]]-[[liaG]]-[[liaF]]-[[liaS]]-[[liaR]]''  
  
* '''[[[[Sigma factor]]]]:''' [[SigA]]
+
* '''Sigma factor:''' [[SigA]]
  
 
* '''Regulation:''' induced by bacitracin ([[LiaR]])
 
* '''Regulation:''' induced by bacitracin ([[LiaR]])

Revision as of 17:50, 15 April 2009

  • Description: negative effector of LiaR

Gene name liaF
Synonyms yvqF
Essential no
Product negative effector of LiaR
Function control of LiaR activity
MW, pI 26 kDa, 9.211
Gene length, protein length 723 bp, 241 aa
Immediate neighbours liaS, liaG
Hier soll was neues rein
Genetic context
YvqF context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: induced by bacitracin (LiaR)
  • Regulatory mechanism: LiaR: transcription activation
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Thorsten Mascher, Karlsruhe Institute of Technology, Germany link

Your additional remarks

References

  1. Jordan, S., A. Junker, J. D. Helmann, and T. Mascher. 2006. Regulation of LiaRS-dependent gene expression in Bacillus subtilis: identification of inhibitor proteins, regulator binding sites, and target genes of a conserved cell envelope stress-sensing two-component system. J. Bacteriol. 188: 5153-5166. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed