Difference between revisions of "Sandbox"

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* '''Description:''' trigger enzyme: sucrose-specific phosphotransferase system, EIIBC component <br/><br/>
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* '''Description:''' trigger enzyme: sucrose-specific phosphotransferase system, EIIBC component (low affinity) <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
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|''sacP''
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|''sacX''
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipa-49d ''
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipa-14r, sacS ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || trigger enzyme: sucrose-specific
+
|style="background:#ABCDEF;" align="center"| '''Product''' || trigger enzyme: sucrose-specific phosphotransferase system, EIIBC component (low affinity)
−
phosphotransferase system, EIIBC component
 
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || sucrose uptake and phosphorylation, control of SacT activity
+
|style="background:#ABCDEF;" align="center"|'''Function''' || sucrose uptake and phosphorylation, control of [[SacY]] activity  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 49 kDa, 7.026  
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 48 kDa, 9.346  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1383 bp, 461 aa  
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1377 bp, 459 aa  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sacA]]'', ''[[ywcJ]]''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[epr]]'', ''[[sacY]]''
 
|-
 
|-
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/sacP_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/sacX_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/sacP_protein.txt Protein sequence]'''
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/sacX_protein.txt Protein sequence]'''
 
|-
 
|-
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|colspan="2" | '''Genetic context''' <br/> [[Image:sacP_context.gif]]
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|colspan="2" | '''Genetic context''' <br/> [[Image:sacX_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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__TOC__
 
__TOC__
  
−
<br/><br/><br/><br/>
+
<br/><br/>
  
 
=The gene=
 
=The gene=
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=== Database entries ===
 
=== Database entries ===
  
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sacPA-ywdA.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sacXY.html]
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10595]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10560]
  
 
=== Additional information===
 
=== Additional information===
Line 82: Line 81:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU38050]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU38410]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 89: Line 88:
  
 
=Expression and regulation=
 
=Expression and regulation=
−
 
−
* '''Operon:''' ''[[sacP]]-[[sacA]]-[[ywdA]]''
 
  
 
* '''Sigma factor:''' [[SigA]]
 
* '''Sigma factor:''' [[SigA]]
  
−
* '''Regulation:''' repressed by glucose ([[CcpA]]),  repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] 
+
* '''Regulation:''' induction by sucrose (at high concentration)
  
−
* '''Regulatory mechanism:''' [[CcpA]]: transcription repression
+
* '''Regulatory mechanism:''' induction by [[SacY]]-dependent RNA switch (transcriptional antitermination)
  
 
* '''Additional information:'''
 
* '''Additional information:'''
Line 121: Line 118:
  
 
# Reizer et al. (1999) Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of ''Bacillus subtilis''.  Microbiology '''145:''' 3419-3429 [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed]
 
# Reizer et al. (1999) Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of ''Bacillus subtilis''.  Microbiology '''145:''' 3419-3429 [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed]
−
# M&#228;der et al. (2002) Transcriptome and Proteome Analysis of ''Bacillus subtilis'' Gene Expression Modulated by Amino Acid Availability. ''J. Bacteriol'' '''184:''' 1844288-4295 [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]
+
# Crutz AM, Steinmetz M, Aymerich S, Richter R, Le Coq D (1990) Induction of levansucrase in Bacillus subtilis: an antitermination mechanism negatively controlled by the phosphotransferase system. J Bacteriol 172:1043-1050. [http://www.ncbi.nlm.nih.gov/sites/entrez/2105292 PubMed]
−
# Fouet, A., Arnaud, M., Klier, A., and G. Rapoport. (1987) ''Bacillus subtilis'' sucrose-specific enzyme II of the phosphotransferase system: Expression in Escherichia coli  and homology to enzymes II from enteric bacteria. Proc Natl Acad Sci USA 84: 8773-8777. [http://www.ncbi.nlm.nih.gov/sites/entrez/3122206 PubMed]
 
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# Débarbouillé, M., Arnaud, M., Fouet, A., Klier, A., and  Rapoport, G. (1990) The sacT gene regulating the ''sacPA'' operon in ''Bacillus subtilis'' shares strong homology with transcriptional antiterminators. J Bacteriol 172: 3966-3973. [http://www.ncbi.nlm.nih.gov/sites/entrez/2163394 PubMed]
 
−
# Arnaud, M., Débarbouillé, M., Rapoport, G., Saier, M. H., and Reizer, J. (1996) In vitro reconstitution of transcriptional antitermination by the SacT and SacY proteins of ''Bacillus subtilis''. J Biol Chem 271: 18966-18972. [http://www.ncbi.nlm.nih.gov/sites/entrez/8702561 PubMed]
 
−
# Sutrina, S. L., Reddy, P., Saier, M. H., Jr & Reizer, J. (1990). The glucose permease of ''Bacillus subtilis'' is a single polypeptide chain that functions to energize the sucrose permease. J Biol Chem 265, 18581-18589. [http://www.ncbi.nlm.nih.gov/sites/entrez/2120236 PubMed]
 
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 11:57, 6 April 2009

  • Description: trigger enzyme: sucrose-specific phosphotransferase system, EIIBC component (low affinity)

Gene name sacX
Synonyms ipa-14r, sacS
Essential no
Product trigger enzyme: sucrose-specific phosphotransferase system, EIIBC component (low affinity)
Function sucrose uptake and phosphorylation, control of SacY activity
MW, pI 48 kDa, 9.346
Gene length, protein length 1377 bp, 459 aa
Immediate neighbours epr, sacY
Gene sequence (+200bp) Protein sequence
Genetic context
SacX context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: PTS permease, sucrose permease (Scr) family PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: induction by sucrose (at high concentration)
  • Regulatory mechanism: induction by SacY-dependent RNA switch (transcriptional antitermination)
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Reizer et al. (1999) Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis. Microbiology 145: 3419-3429 PubMed
  2. Crutz AM, Steinmetz M, Aymerich S, Richter R, Le Coq D (1990) Induction of levansucrase in Bacillus subtilis: an antitermination mechanism negatively controlled by the phosphotransferase system. J Bacteriol 172:1043-1050. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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