Difference between revisions of "Sandbox"

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* '''Description:''' repression of the hexuronate utilization operon (uxaC-yjmBCD-uxuA-yjmf-exuTR-uxaBA) <br/><br/>
+
* '''Description:''' tagaturonate reductase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''exuR''
+
|''uxaB''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yjmH ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yjmI ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional repressor (LacI family)
+
|style="background:#ABCDEF;" align="center"| '''Product''' || tagaturonate reductase
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of hexuronate utilization
+
|style="background:#ABCDEF;" align="center"|'''Function''' || hexuronate utilization  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 5.846  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 55 kDa, 5.056  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 999 bp, 333 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1440 bp, 480 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[exuT]]'', ''[[uxaB]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[exuR]]'', ''[[uxaA]]''
 
|-
 
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/exuR_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/uxaB_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/exuR_protein.txt Protein sequence]'''
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/uxaB_protein.txt Protein sequence]'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:exuR_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:uxaB_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/uxaC-yjmBCD-uxuA-yjmF-exuTR-uxaBA.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/uxaC-yjmBCD-uxuA-yjmF-exuTR-uxaBA.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13211]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13212]
  
 
=== Additional information===
 
=== Additional information===
Line 81: Line 81:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU12370]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU12380]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''

Revision as of 00:49, 3 April 2009

  • Description: tagaturonate reductase

Gene name uxaB
Synonyms yjmI
Essential no
Product tagaturonate reductase
Function hexuronate utilization
MW, pI 55 kDa, 5.056
Gene length, protein length 1440 bp, 480 aa
Immediate neighbours exuR, uxaA
Gene sequence (+200bp) Protein sequence
Genetic context
UxaB context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation: repressed by glucose (CcpA)
  • Regulatory mechanism: CcpA: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed