Difference between revisions of "Sandbox"

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|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''mrpA''
+
|''bglP''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yufT, shaA, ntrA ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''sytA ''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
+
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || Na+/H+ antiporter
+
|style="background:#ABCDEF;" align="center"| '''Product''' || phosphotransferase system (PTS) beta-glucoside-specific enzyme IIBCA
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Function''' || multiple resistance and pH homeostasis
+
|style="background:#ABCDEF;" align="center"|'''Function''' || beta-glucosides uptake
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 86 kDa, 9.519  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 64 kDa, 6.809  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2322 bp, 774 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1827 bp, 609 aa  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yufS]]'', ''[[mrpB]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[bglH]]'', ''[[yxxE]]''
 
|-
 
|-
−
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/mrpA_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/bglP_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
−
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/mrpA_protein.txt Protein sequence]'''
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/bglP_protein.txt Protein sequence]'''
 
|-
 
|-
−
|colspan="2" | '''Genetic context''' <br/> [[Image:mrpA_context.gif]]
+
|colspan="2" style="background:#FAF8CC;color:#FF0000" align="center" | '''Caution: The sequence for this gene in SubtiList contains errors
 +
|-
 +
|colspan="2" | '''Genetic context''' <br/> [[Image:bglP_context.gif]]
 
|-
 
|-
 
|}
 
|}
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
−
 
−
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
 
  
 
=== Database entries ===
 
=== Database entries ===
  
−
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mrpABCDEFG.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/bglPH-yxiE.html]
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12355]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10934]
  
 
=== Additional information===
 
=== Additional information===
Line 84: Line 84:
 
* '''KEGG entry:'''
 
* '''KEGG entry:'''
  
−
* '''E.C. number:'''
+
* '''E.C. number:''' 2.7.1.69
  
 
=== Additional information===
 
=== Additional information===
Line 90: Line 90:
 
=Expression and regulation=
 
=Expression and regulation=
  
−
* '''Operon:'''  
+
* '''Operon:''' ''[[bglP]]-[[bglH]]-[[yxiE]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/7883710 PubMed]
  
−
* '''Sigma factor:'''  
+
* '''Sigma factor:''' [[SigA]]
  
−
* '''Regulation:'''  
+
* '''Regulation:''' induced by salicin  [http://www.ncbi.nlm.nih.gov/sites/entrez/7883710 PubMed], repressed by glucose (catabolite repression)
  
−
* '''Regulatory mechanism:'''  
+
* '''Regulatory mechanism:''' Induction: [[LicT]]-dependent RNA switch (antitermination), catabolite repression: repression by [[CcpA]]  ([[CcpA]] binding site overlaps -35 region) and lack of [[LicT]]-dependent antitermination in the presence of gucose due to the requirement of [[LicT]] to be phosphorylated by [[PtsH| HPr]]
  
−
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =
Line 120: Line 120:
 
=References=
 
=References=
  
 +
# Le Coq, D., Lindner, C., Krüger, S., Steinmetz, M. & Stülke, J. (1995) New ß-glucosides (bgl) genes in ''Bacillus subtilis'': The ''bglP'' gene product has both transport and regulatory functions, similar to that of the ''Escherichia coli'' BglF protein. J. Bacteriol. 177: 1527-1535.  [http://www.ncbi.nlm.nih.gov/sites/entrez/7883710 PubMed]
 +
# Krüger, S., Gertz, S. & Hecker, M. Transcriptional analysis of ''bglPH'' expression in ''Bacillus subtilis'': Evidence for two distinct pathways mediating carbon catabolite repression. J. Bacteriol. 178, 2637-2644 (1996). [http://www.ncbi.nlm.nih.gov/sites/entrez/8626332 PubMed]
 +
# Krüger, S. and Hecker, M. (1995) Regulation of the putative ''bglPH'' operon for aryl-ß-glycoside utilization in ''Bacillus subtilis''. J. Bacteriol. 177, 5590-5597. [http://www.ncbi.nlm.nih.gov/sites/entrez/7559347 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 16:54, 9 March 2009

  • Description: write here

Gene name bglP
Synonyms sytA
Essential no
Product phosphotransferase system (PTS) beta-glucoside-specific enzyme IIBCA
Function beta-glucosides uptake
MW, pI 64 kDa, 6.809
Gene length, protein length 1827 bp, 609 aa
Immediate neighbours bglH, yxxE
Gene sequence (+200bp) Protein sequence
Caution: The sequence for this gene in SubtiList contains errors
Genetic context
BglP context.gif



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry:
  • E.C. number: 2.7.1.69

Additional information

Expression and regulation

  • Regulation: induced by salicin PubMed, repressed by glucose (catabolite repression)
  • Regulatory mechanism: Induction: LicT-dependent RNA switch (antitermination), catabolite repression: repression by CcpA (CcpA binding site overlaps -35 region) and lack of LicT-dependent antitermination in the presence of gucose due to the requirement of LicT to be phosphorylated by HPr
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Le Coq, D., Lindner, C., Krüger, S., Steinmetz, M. & Stülke, J. (1995) New ß-glucosides (bgl) genes in Bacillus subtilis: The bglP gene product has both transport and regulatory functions, similar to that of the Escherichia coli BglF protein. J. Bacteriol. 177: 1527-1535. PubMed
  2. Krüger, S., Gertz, S. & Hecker, M. Transcriptional analysis of bglPH expression in Bacillus subtilis: Evidence for two distinct pathways mediating carbon catabolite repression. J. Bacteriol. 178, 2637-2644 (1996). PubMed
  3. Krüger, S. and Hecker, M. (1995) Regulation of the putative bglPH operon for aryl-ß-glycoside utilization in Bacillus subtilis. J. Bacteriol. 177, 5590-5597. PubMed
  4. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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