Difference between revisions of "Abn2"
| Line 4: | Line 4: | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Gene name''' | |style="background:#ABCDEF;" align="center"|'''Gene name''' | ||
| − | |'' | + | |''abn2'' |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' '' | + | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yxiA '' |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
| Line 62: | Line 62: | ||
=== Extended information on the protein === | === Extended information on the protein === | ||
| − | * '''Kinetic information:''' | + | * '''Kinetic information:''' With linear-alpha-1,5-l-arabinan as the preferred substrate, the enzyme exhibited an apparent K(m) of 2.0 mg ml(-1) and V(max) of 0.25 mmol min(-1) mg(-1) at pH 7.0 and 50°C. [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed] |
* '''Domains:''' | * '''Domains:''' | ||
| Line 74: | Line 74: | ||
* '''Interactions:''' | * '''Interactions:''' | ||
| − | * '''Localization:''' | + | * '''Localization:''' extracellular [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed] |
=== Database entries === | === Database entries === | ||
| Line 84: | Line 84: | ||
* '''KEGG entry:''' | * '''KEGG entry:''' | ||
| − | * '''E.C. number:''' | + | * '''E.C. number:''' 3.2.1.99 |
=== Additional information=== | === Additional information=== | ||
| Line 90: | Line 90: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' ''abn2'' [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed] |
| − | * '''Sigma factor:''' | + | * '''Sigma factor:''' [[SigA]], [[SigH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed] |
| − | * '''Regulation:''' | + | * '''Regulation:''' expression is stimulated by arabinose and pectin and repressed by glucose [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed] |
| − | * '''Regulatory mechanism:''' | + | * '''Regulatory mechanism:''' unknown [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed] |
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 115: | Line 115: | ||
=Labs working on this gene/protein= | =Labs working on this gene/protein= | ||
| + | |||
| + | [[Isabel de Sa-Nogueira]] | ||
=Your additional remarks= | =Your additional remarks= | ||
| + | |||
=References= | =References= | ||
| + | # Inácio JM, and de Sá-Nogueira I(2008) Characterization of ''abn2'' (''yxiA''), encoding a ''Bacillus subtilis'' GH43 arabinanase, Abn2, and its role in arabino-polysaccharide degradation.''J. Bacteriol.'' '''190:''' 4272-4280. [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed] | ||
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed] | # Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed] | ||
Revision as of 13:04, 18 March 2009
- Description: write here
| Gene name | abn2 |
| Synonyms | yxiA |
| Essential | no |
| Product | |
| Function | unknown |
| MW, pI | 52 kDa, 7.371 |
| Gene length, protein length | 1407 bp, 469 aa |
| Immediate neighbours | yxiB, hutP |
| Gene sequence (+200bp) | Protein sequence |
| Caution: The sequence for this gene in SubtiList contains errors | |
Genetic context 
| |
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: With linear-alpha-1,5-l-arabinan as the preferred substrate, the enzyme exhibited an apparent K(m) of 2.0 mg ml(-1) and V(max) of 0.25 mmol min(-1) mg(-1) at pH 7.0 and 50°C. PubMed
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: extracellular PubMed
Database entries
- Structure:
- Swiss prot entry:
- KEGG entry:
- E.C. number: 3.2.1.99
Additional information
Expression and regulation
- Operon: abn2 PubMed
- Regulation: expression is stimulated by arabinose and pectin and repressed by glucose PubMed
- Regulatory mechanism: unknown PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
