RecF

• Description: promoter of RecA DNA repair center assembly
  
  

Gene name	recF
Synonyms
Essential
Product	promoter of RecA DNA repair center assembly
Function	DNA repair/ recombination
Gene expression levels in SubtiExpress: RecF
MW, pI	42 kDa, 7.015
Gene length, protein length	1110 bp, 370 aa
Immediate neighbours	yaaA, yaaB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

The gene

Basic information

• Locus tag: BSU00040

Phenotypes of a mutant

• drastically reduced survival of mature dormant spores after exposure to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs) PubMed

Database entries

• BsubCyc: BSU00040

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • increases the efficiency of RecA DNA repair center assembly PubMed

• Protein family: recF family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU00040

• Structure:

• UniProt: P05651

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yaaA-recF-yaaB-gyrB PubMed

• Expression browser: recF PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

Original publications

Justin S Lenhart, Eileen R Brandes, Jeremy W Schroeder, Roderick J Sorenson, Hollis D Showalter, Lyle A Simmons
RecO and RecR are necessary for RecA loading in response to DNA damage and replication fork stress.
J Bacteriol: 2014, 196(15);2851-60
[PubMed:24891441] [WorldCat.org] [DOI] (I p)

Ignacija Vlašić, Ramona Mertens, Elena M Seco, Begoña Carrasco, Silvia Ayora, Günther Reitz, Fabian M Commichau, Juan C Alonso, Ralf Moeller
Bacillus subtilis RecA and its accessory factors, RecF, RecO, RecR and RecX, are required for spore resistance to DNA double-strand break.
Nucleic Acids Res: 2014, 42(4);2295-307
[PubMed:24285298] [WorldCat.org] [DOI] (I p)

Dawit Kidane, Humberto Sanchez, Juan C Alonso, Peter L Graumann
Visualization of DNA double-strand break repair in live bacteria reveals dynamic recruitment of Bacillus subtilis RecF, RecO and RecN proteins to distinct sites on the nucleoids.
Mol Microbiol: 2004, 52(6);1627-39
[PubMed:15186413] [WorldCat.org] [DOI] (P p)

S Ayora, J C Alonso
Purification and characterization of the RecF protein from Bacillus subtilis 168.
Nucleic Acids Res: 1997, 25(14);2766-72
[PubMed:9207023] [WorldCat.org] [DOI] (P p)

J C Alonso, A C Stiege, G Lüder
Genetic recombination in Bacillus subtilis 168: effect of recN, recF, recH and addAB mutations on DNA repair and recombination.
Mol Gen Genet: 1993, 239(1-2);129-36
[PubMed:8510642] [WorldCat.org] [DOI] (P p)

J C Alonso, A C Stiege
Molecular analysis of the Bacillus subtilis recF function.
Mol Gen Genet: 1991, 228(3);393-400
[PubMed:1716726] [WorldCat.org] [DOI] (P p)

N Ogasawara, S Moriya, H Yoshikawa
Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. IV. Transcription of the oriC region and expression of DNA gyrase genes and other open reading frames.
Nucleic Acids Res: 1985, 13(7);2267-79
[PubMed:2987848] [WorldCat.org] [DOI] (P p)