RadA

• Description: 6-O-methylguanine-DNA methyltransferase, negative effector of DisA activity, participates in the stabilization and/or processing of holliday junction intermediates
  
  

Gene name	radA
Synonyms	sms, yacJ
Essential	no
Product	6-O-methylguanine-DNA methyltransferase
Function	homologous recombination , control of c-di-AMP formation
Gene expression levels in SubtiExpress: radA
Interactions involving this protein in SubtInteract: RadA
Metabolic function and regulation of this protein in SubtiPathways: radA
MW, pI	49 kDa, 8.128
Gene length, protein length	1374 bp, 458 aa
Immediate neighbours	clpC, disA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

metabolism of signalling nucleotides, DNA repair/ recombination, general stress proteins (controlled by SigB), heat shock proteins

This gene is a member of the following regulons

CtsR regulon, SigB regulon

The gene

Basic information

• Locus tag: BSU00870

Database entries

• BsubCyc: BSU00870

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: RadA subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • RadA-DisA PubMed

• Localization:

Database entries

• BsubCyc: BSU00870

• Structure:

• UniProt: P37572

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ctsR-mcsA-mcsB-clpC-radA-disA PubMed

• Expression browser: radA PubMed

• Sigma factor: SigA PubMed, SigB PubMed1 PubMed2

• Regulation: induced by stress (SigB) PubMed, induced by heat (CtsR) PubMed

• Regulatory mechanism: CtsR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • BKG1 (radA::spc), available in Jörg Stülke's lab
  • BKG2 (radA-disA::spc), available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: